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        Subtractive cDNA Cloning Using Magnetic Beads and PCR

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        Subtractive cloning is a method that facilitates the isolation of nucleotide sequences present in a test sample but absent or present at much lower levels, in a reference sample. A variety of situations lend themselves to this methodology and it has been used, for example, to characterize changes in levels of gene expression during development (1 3 ), in pathological situations (4 6 ), in inductive events (7 ,8 ), and for the isolation of tissue-specific genes (1 ,9 11 ). Current methodologies also allow detection of pathogens and integrated retroviruses present at exceedingly low levels in the cell (12 ).
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