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- 用户评价
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
3个月
- 英文名:
SARS-CoV-2 (2019-nCoV) Spike Gene ORF cDNA clone expression plasmid (Codon Optimized)
- 库存:
999
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1 Unit
SARS-CoV-2 (2019-nCoV) Spike ORF mammalian expression plasmid (Codon Optimized): 产品信息
基因
种属 2019-nCoV
基因长度 3822 bp
序列特征 A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system.The translated amino acid sequence is identical with QHD43416.1
产品特征 Full length Clone DNA of SARS-CoV-2 (2019-nCoV) Spike.
质粒
启动子 Enhanced CMV mammalian cell promoter
载体 pCMV3-untagged
限制性酶切位点 KpnI + XbaI(6.1kb+3.82kb)
测序引物 T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
质控 The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性 Ampicillin
细胞筛选抗性 Hygromycin
应用 Stable or Transient mammalian expression
北京义翘神州科技股份有限公司(Sino Biological Inc.)创建于2007年,是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司,主要业务包括重组蛋白、抗体、基因和培养基等产品,以及重组蛋白、抗体的开发和生物分析检测等服务。
义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务,能够覆盖生命科学研究的多个领域,为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供“一站式”生物试剂产品和技术服务。客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲等地建立了子公司,在上海建立分公司,已成为生物试剂行业国内领先的科技公司之一。
凭借多年病毒蛋白研究技术优势,义翘神州仅用11天就成功表达出新冠病毒重要靶点受体结合域蛋白。为了更好的支持新冠病毒研究,义翘神州已开发生产近百种新冠病毒相关科研试剂,包括重要靶点抗原蛋白、中和抗体、抗原蛋白检测试剂盒等。义翘神州还可以提供新冠假病毒中和检测服务,可用于筛选出具有中和活性的抗体或血清,支持在细胞水平上的评估。目前,这些科研试剂已被全球数百家研究机构用于疫苗和抗体开发及基础机制研究。
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文献和实验- Xiang-Jiao Yang, et al. P3 site-directed mutagenesis: An efficient method based on primer pairs with 3'-overhangs. The Journal of biological chemistry, PubMed ID: 39863101
- Min-Suk Song, et al. Fc-binding nanodisc restores antiviral efficacy of antibodies with reduced neutralizing effects against evolving SARS-CoV-2 variants. Journal of nanobiotechnology, PubMed ID: 39856746
- Lu M, et al. Real-time conformational dynamics of SARS-CoV-2 spikes on virus particles.bioRxiv : the preprint server for biology, PubMed ID: 32935100
- Ozono S, et al. SARS-CoV-2 D614G spike mutation increases entry efficiency with enhanced ACE2-binding affinity.Nature communications, PubMed ID: 33558493
- Harrington WE, et al. Rapid decline of neutralizing antibodies is associated with decay of IgM in adults recovered from mild COVID-19 disease.Cell Reports Medicine, PubMed ID: 33842901
- Kaku Y, et al. Resistance of SARS-CoV-2 variants to neutralization by antibodies induced in convalescent patients with COVID-19.Cell reports, PubMed ID: 34237284
- Izumida M, et al. Unique Mode of Antiviral Action of a Marine Alkaloid against Ebola Virus and SARS-CoV-2.Viruses, PubMed ID: 35458549
- Okura T, et al. Hydrophobic Alpha-Helical Short Peptides in Overlapping Reading Frames of the Coronavirus Genome.Pathogens (Basel, Switzerland), PubMed ID: 36014999
- Cao X, et al. Unbiased approach to identify and assess efficacy of human SARS-CoV-2 neutralizing antibodies.Scientific reports, PubMed ID: 36109550
- Chen Y, et al. Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD.iScience, PubMed ID: 36514310
- Elste J, et al. Co-Expression of Niemann-Pick Type C1-Like1 (NPC1L1) with ACE2 Receptor Synergistically Enhances SARS-CoV-2 Entry and Fusion.Biomedicines, PubMed ID: 38672177
- Kumar P, et al. Identification of antibody-resistant SARS-CoV-2 mutants via N4-Hydroxycytidine mutagenesis.Antiviral research, PubMed ID: 39293594
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