Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
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We previously isolated Aurora-C (Aurkc/Aie1
) in a screen for kinases expressed in mouse sperm and eggs. Aurora-C kinase was reported to be a chromosomal passenger protein
that plays critical roles in chromosome alignment, segregation, kinetochore-microtubule attachment, and cytokinesis in female
mouse meiosis. This chapter describes experimental approaches for examining the subcellular localization and function of Aurora-C
kinase during female mouse meiosis, presenting detailed methods for introducing exogenous Aurora-C wild-type and kinase-dead
mutant mRNAs into mouse oocytes by cytosolic microinjection, and preparing whole-mount meiotic oocytes and chromosome spreads
for confocal immunofluorescence microscopy.
