Studying Meiosis-Specific Cohesins in Mouse Embryonic Oocytes
互联网
676
Distinct meiotic cohesin complexes play fundamental roles in various meiosis-specific chromosomal events in spatiotemporally
different manners during mammalian meiotic prophase. Immunostaining is one of the essential methods to study meiotic cohesin
dynamics. For the study of cohesins in the meiotic prophase of oocytes, ovaries should be taken from the embryos during a
very limited period before birth. Here we focus on some technical tips concerning the preparation of oocyte chromosome spreads
for immunostaining. Further, we describe a method for chromosome fluorescence in situ hybridization (FISH) against immunostained
oocytes.
