• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Production of Hybridization Probes by the PCR Utilizing Digoxigenin-Modified Nucleotides

        互联网

        428
        The polymerase chain reaction (PCR) (1) is an efficient method for copying a fragment of DNA when primers exist that flank the target sequence. We have found that since most cloning vectors utilize the lacZ gene with a synthetic multiple cloning site (2), a single pair of primers will amplify sequences inserted into many common phage and plasmid vectors, such as the pUC plasmid and Lambda Zap phage series and their derivatives. We have had little difficulty amplifying inserts up to 3000 bp in length and now consider this as a preferred alternative to growth of bacterial cultures with subsequent nucleic acid purification to produce DNA fragments of this length for both mapping and sequencing.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序