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        一些关于PCR的网站

        丁香园论坛

        3255

        一个PCR相关的专业网站,内容很全
        http://www.pcrlinks.com/

        实验方法与步骤详解
        http://swjsx.ntmc.edu.cn/Protocol/home.htm

        美国AXYgen公司
        http://www.axygen.com/NewFiles/pcr.html

        http://www.alkami.com/
        This 158 page PCR manual covers the following topics
        PCR Primer design
        PCR Methods
        PCR Polymerases
        PCR Variables
        PCR Troubleshooting
        Special PCR Topics
        Appendixes and a comprehensive Index

        BioGuide - PCR
        http://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html
        魏茨曼基因组和生物信息学研究所的一个网页,介绍PCR的初步知识。
        包括:
        What is PCR?
        What are some good reference books for PCR?
        How should I select a set of primers to use for PCR?
        Programs for designing PCR primers
        What is "Hot-start" PCR?
        What is AP-PCR or RAPD PCR?
        What is "Touchdown" PCR?
        Is there a simple method to sequence lambda, M13, or plasmid clones using PCR?

        A Look at PCR Cloning Tools
        http://www.bioon.com/bbs/post/view?bid=67&id=151321&sty=1&tpg=63&age=0

        PCR常见问题及回答
        http://www.invitrogen.com.cn/techtitle/main/faq_PCR.htm#2

        PCR和Realtime PCR的原理讲的很透, 一个介绍Realtime PCR的教学网站,里面的powerpoint文件非常好
        http://www.med.sc.edu:85/pcr/realtime-home.htm

        SYBR Green PCR
        http://www.bioon.com/bbs/post/view?bid=67&id=638781&sty=1&tpg=5&ppg=1&age=0#638781

        PCR QUICK GUIDE
        http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdfhttp://www.sfb363.uni-halle.de/mod-path/differential_display/sturtevant00.pdfhttp://www.wooster.edu/biology/courses/bio309/default.html

        PCR拼接
        http://www.bioon.com/bbs/post/view?bid=67&id=305337&sty=1&tpg=47&age=0

        知道蛋白如何寻找其氨基酸序列
        http://www.bioon.com/bbs/post/view?bid=67&id=401195&sty=1&tpg=36&age=0
        http://us.expasy.org/cgi-bin/niceprot.pl?P08253

        关于DNA的flash版和文字版
        http://www.pbs.org/wgbh/nova/genome/dna.html#

        ABI Prism 7000 简要使用说明
        http://www.genomultix.com/tech/7000.htm

        好书推荐:Practical Protocols of Gene Engineering
        http://www.estarray.org/books/

        http://www.life.uiuc.edu/bio100/lectures/s02lects/18s02biotechclonerecomb.html
        Website dealing with cloning and molecular analysis of genes
        http://www.ndsu.nodak.edu/instruct/mcclean/plsc431/cloning/clone3.htm
        Some really wonderful illustrations of the techniques of genetic engineering!
        http://www.uic.edu/classes/phar/phar331/lecture6/
        A truly amazing global resource page on cloning, stem cell research, and related ethical issues, including recent news articles and legislative updates
        http://genomics.phrma.org/cloning.html
        Animation and article on interspecies cloning
        http://www.brookscole.com/chemistry_d/templates/student_resources/0030244269_campbell/HotTopics/InterspeciesCloning.html
        From PBS...an animation that illustrates how stem cells are cultivated
        http://www.pbs.org/newshour/health/stem_cells.swf
        Animations illustrating the use of viruses as vectors for gene therapy
        http://www.technologyreview.com/articles/5940.htm
        Animations of techniques used for cloning mammals, as well as a create-your-own-clone link
        http://library.thinkquest.org/24355/data/details/media/index.html
        Another animation of PCR
        http://www.people.virginia.edu/~rjh9u/pcranim.html
        Animations of restriction digests and gel electrophoresis
        http://www.biology.ucsc.edu/classes/bio20L/animate

        Gel electrophoresis techniques and simulations
        A wonderful website that includes links to a virtual lab on agarose gel electrophoresis:
        http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/
        A Massachusetts Institute of Technology website dealing with Southerns, Northerns, Westerns, and cloning:
        http://web.mit.edu/esgbio/www/rdna/rdna.html
        2-D gel electrophoresis of proteins:
        http://www.fitzscipress.com/Chap05.pdf
        A website containing lots of links to electrophoresis techniques and simulations:
        http://biobenchelper.hypermart.net/pr/electrophoresis.htm
        Want to learn more about chemical bonds? Check out this website:
        http://hyperphysics.phy-astr.gsu.edu/hbase/chemical/bond.html

        多个录象-多个著名公司
        http://www.biocompare.com/video.asp

        PCR和 RFLP动画片
        http://medtech.cls.msu.edu/ISL/immunology/pcr.htmhttp://medtech.cls.msu.edu/ISL/immunology/rflp.htm

        redasoft visual cloning 2000 patch
        http://www.bioon.com/bbs/post/view?bid=67&id=496454&sty=1&tpg=26&age=0

        分子生物学屏幕保护程序下载
        pcr
        http://biosoft.biosino.org/down/PCR.exe
        蛋白
        http://biosoft.biosion.org/down/ssaver.zip
        amavi100
        http://ourworld.compuserve.com/homepages/esmsoftware/amavi100.zip
        dna旋转
        http://www.rocketdownload.com/dnload/dna.exe
        ddnabase
        http://www.rocketdownload.com/dnload/ddnabase.exe
        cyclovir
        http://mama.indstate.edu/pfaffle/software/cyclovir.exe

        http://biosoft.biosino.org/down/source.exehttp://ftp.monash.edu.au//pub/win.galttech/gtmolecules.zip

        分子生物学相关软件
        http://gene.tmmu.com.cn/biosoft/content.html
        1.DNA分析:数据库GenBank, EMBL, DDBJ 查询软件;用来帮助进行PCR引物设计与基因探针设计的软件;功能很全面的DNA序列分析工具包;DNA分析与绘图软件,可绘制线性或环形DNA图;限制酶消化工具;以Java语言写成的序列比较查看器
        2.RNA分析:RNA二级结构分析软件;预测RNA二级结构图
        3.蛋白质分析:蛋白序列分析软件包;研究膜蛋白拓扑学结构和其它特性的JAVA软件
        4.生化教学:生化基础概念演示教学程序; 生化教学文件
        5.生化工程:生物反应器(发酵罐)设计软件;发酵实时模拟软件;计算机辅助设计特别是生物工艺设计的软件包
        6.序列格式转换:FASTA与BLAST查询输出文件的处理软件
        7.引物分析
        8.序列综合分析
        9.检索与阅读
        10.基因芯片

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