生物信息学技术

Abstract Table of Contents Literature Cited Abstract In the mid? to late 1990s, because of the drug discovery paradigm shift from phenotypic screens to combinatorial chemistry and high?throughput screening, the physicochemical properties of exploratory drug molecules displa

Abstract Table of Contents Figures Literature Cited Abstract Nucleotide analogues represent a major class of anti?cancer and anti?viral drugs, and provide an extremely powerful tool for dissecting the mechanisms of DNA and RNA polymerases.

Abstract Table of Contents Materials Figures Literature Cited Abstract Measuring the rate and extent of radioligand binding provides information on the number of binding sites, and their affinity and acce

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes ChIP?Seq methodology, which involves chromatin immunoprecipitation (ChIP) followed by high?throughput sequencing

Abstract Table of Contents Materials Figures Literature Cited Abstract Genetically encodable FRET?based kinase activity reporters (KARs) enable real?time monitoring of kinase activity dynamics in living c

Abstract Table of Contents Figures Literature Cited Abstract This unit describes critical components and considerations required to study protein?protein structural interactions inside a living cell by using NMR spectroscopy (STINT?NMR). S

Abstract Table of Contents Materials Figures Literature Cited Abstract Hepatitis C virus (HCV) affects an estimated 3% of the population and is a leading cause of chronic liver disease worldwide. Since HC

Abstract Table of Contents Materials Figures Literature Cited Abstract The kinetics of the ligand?receptor interaction is an important feature in lead optimization for new drug candidates. The protocol de

Abstract Table of Contents Materials Figures Literature Cited Abstract Peptide?oligonucleotide conjugates (POCs) are molecular chimeras composed of a nucleic acid moiety covalently attached to a polypepti

Abstract Table of Contents Materials Figures Literature Cited Abstract The detailed synthetic protocol for a 2?selenothymidine phosphoramidite and its use in preparing Se?derivatized oligonucleotides are

Abstract Table of Contents Materials Figures Literature Cited Abstract Metabolic oligosaccharide engineering (MOE) refers to a technique where non?natural monosaccharide analogs are introduced into living

Abstract Table of Contents Literature Cited Abstract Inhibitors of specific cellular pathways are useful for investigating the roles of proteins of unknown function, and for selectively inhibiting a protein in complex pathways to uncover its relationships to other proteins

Abstract Table of Contents Materials Figures Literature Cited Abstract Recent development of methods for genome?wide identification of transcription factor binding sites by chromatin immunoprecipitation (

Abstract Table of Contents Figures Literature Cited Abstract In addition to the orthosteric site, which recognizes endogenous ligands, most G protein?coupled receptors (GPCRs) possess topographically distinct allosteric sites that can be r

Abstract Table of Contents Materials Figures Literature Cited Abstract Cyclic nucleoside phosphonates connected through a P?O?C linkage to a promoiety represent a class of prodrugs designed to overcome th

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the preparation of alkylthioalkylated and formamidoalkylated alcohols, an amidoalkylated alcohol, a hydroxylalkylat

Abstract Table of Contents Materials Figures Literature Cited Supplementary Material Abstract Supported membranes, formed as a single conti

Abstract Table of Contents Materials Figures Literature Cited Abstract Imaging of RNAs in single cells revealed their localized transcription and specific function. Such information cannot be obtained fro

Abstract Table of Contents Materials Figures Literature Cited Abstract Allosteric interactions involve the simultaneous binding of two ligands to the same receptor. An allosteric modulator causes a confor

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a simple procedure for preparing short DNA duplexes that contain a single N4C?alkyl?N4C interstrand cross?link. The