蛋白质技术

ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION P.J. Hansen Dept. of Animal Sciences University of Florida INTRODUCTIONImmunoprecipitation is a procedure by which peptides or proteins that react specifica ...

Antibody Precipitation with Protein A or GAuthor: James TurmoSource: Contributed by James TurmoAbstract: Using Immobilized Protein A or Protein G for Immunoprecipitation.PurposeUsing Immobilized Prote ...

CO-IMMUNOPRECIPITATION ANALYSIS OF PROTEIN-PROTEIN INTERACTIONSThis protocol uses total cell extracts to analyze putative protein-protein interactions in eukaryotic cells. One can also use nuclear ex ...

CO-IMMUNOPRECIPITATION ANALYSIS OF PROTEIN-PROTEIN INTERACTIONSThis protocol uses total cell extracts to analyze putative protein-protein interactions in eukaryotic cells. One can also use nuclear ex ...

Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley 1) Wash cells with cold "Tris" (from Margie's shelf).Spin down the cells if you are working with suspension cells in a ...

Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley 1) Wash cells with cold "Tris" (from Margie's shelf).Spin down the cells if you are working with suspension cells in a ...

Immunoprecipitation of metabolically labeled cells(Protocol from An Zhou Johns Hopkins 1995) 1. To your sample add Super E protease inhibitors 1 mM cold Met antibody. TMT extract or medium - dilute ...

Immunoprecipitation of metabolically labeled cells(Protocol from An Zhou Johns Hopkins 1995) 1. To your sample add Super E protease inhibitors 1 mM cold Met antibody. TMT extract or medium - dilute ...

Preparation of Modifed Radioimmunoprecipitation (RIPA) BufferRIPA Base IngredientsTris-HCl (buffering agent prevents protein denaturation) NaCl (salt prevents non-specific protein aggregation) NP-40 ( ...

Western Blotting or immunoblotting (Western blotting was dubbed because it is similar to Southern blotting which was invented by and named after the inventor E. M. Southern) allows investigators to de ...

Bacterial Protein Extraction (mini-scale) Using B-Per B-Per (Pierce Cat number: 78248) Extraction of 1.5ml bact.culture OD600nm 1.5-3.5A useful procedure for initial screening of expression conditions ...

Western Blotting or immunoblotting (Western blotting was dubbed because it is similar to Southern blotting which was invented by and named after the inventor E. M. Southern) allows investigators to de ...

WESTERN PROTOCOLA. Preparation of cell lysates Collect cells (confluent T-25) by trypsinization and spin. Lyse the pellet with 100 µl lysis buffer on ice for 10 min. For 500000 cells lyse with 20 ...

Western Blot MaterialsSemi-dry Transfer ApparatusBiorad Cat# 170-3940 or equivalentPower Supply0 - 100 VDC (adj. current to 1 Amp)Immobilon-P transfer membrane0.45 µm pore size; cut to same size as g ...

Cell Lysates For Western Blotting Reagents / Solutions Lysis Buffer:10ml 10% Sodium dodecyl sulphate (SDS)10ml Glycerol10ml b-mercaptoethanol8ml 0.5M Tris pH6.81ml 0.1% bromophenol blue51ml H2O Proto ...

Cell Lysates For Western Blotting Reagents / Solutions Lysis Buffer:10ml 10% Sodium dodecyl sulphate (SDS)10ml Glycerol10ml b-mercaptoethanol8ml 0.5M Tris pH6.81ml 0.1% bromophenol blue51ml H2O Proto ...

Western Blot 1) Run protein gel.Set up blot:2) Prepare two tupperware containers one with dH2O and one with 1/2X Transfer Buffer (TB).3) Remove one glass plate from the gel by twisting a spacer betwee ...

REAGENTS

Western Blotting with Alkaline Phosphatase ConjugatesSAMPLE PREPARATIONFor Protein Concentration Determination of Cell CultureDecant medium from 10cm dish of adherent cells and rinse plate rapidly wit ...

SAMPLE PREPARATIONFor Protein Concentration Determination of Cell CultureDecant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS). Aspirate excess PB ...