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文献和实验, such as parallel‐line grid patterns, are then manufactured by a mask‐making vendor) Metal target (99.99% chromium, Alfa Aesar) Thin film evaporator (Edwards EB3 electron beam evaporator; Edwards) Additional
), and boil samples for 3-5min. 16.) Run samples on 12% or 15% gel. Stain gel, dry and expose to film or Phosphorimager screen. I usually load between 20 and 30 μl, and I can see a strong signal overnight. Sample can also be stored, after boiling, at -20oC
Functional Assays on High‐Content Protein Microarrays
Thick filter paper X‐ray film cassette Tritium‐sensitive phosphor imager screen (PerkinElmer, cat
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