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文献和实验v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
for 1.0 M NaCl; and 60% solution Bfor 1.2 M NaCl). 2.3. Chelating Chromatography 1. Hi-Trap chelating affinity column (1.0-mL bed volume, GEHealthcare). 2. 0.1 M nickel sulfate (NiSO 4 ). 3. Equilibration solution: 0.05 M NaPO 4 and 0.01 M
Measurement of Nitric Oxide in Single Cells and Tissue Using a Porphyrinic Microsensor
of a conducting polymeric metalloporphyrin catalyst, overlaid with another thin film of Nafion, a cation exchange material. The electric current generated during NO oxidation on the surface of the polymeric porphyrin is linearly proportional to the concentration
Studies of the Ubiquitin Proteasome System
Biomedicals Life Science Division, or see recipe ) or HRP‐conjugated anti–rabbit Ig and chemiluminescent detection reagents (SuperSignal West Pico, Pierce, or ECL Plus, GE Healthcare) Biomax MR film (Kodak
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