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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
SARS-CoV-2 (2019-nCoV) Spike RBD (Y453F) Protein (rFc Tag)
- 保质期:
12个月
- 供应商:
北京义翘神州科技股份有限公司
- 保存条件:
-20℃ to -80℃
- 规格:
100 µg
纯度> 95 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Testing in progress
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD (YP_009724390.1, with mutation Y453F) (Arg319-Phe541) was expressed with the Fc region of rabbit IgG1 at the C-terminus.
表达宿主HEK293 Cells
种属SARS-CoV-2
预测 N 端Ser
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD consists of 448 amino acids and predicts a molecular mass of 50.4 kDa. As a result of glycosylation, it migrates as an approximately 53 kDa band in SDS-PAGE under reducing conditions.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验日前,北京大学生命科学学院魏文胜课题组在 Cell 杂志上在线发表题为“Circular RNA Vaccines against SARS-CoV-2 and Emerging Variants”的研究论文。 魏文胜团队首先建立了体外高效制备高纯度环状 RNA 的技术平台,针对新型冠状病毒及其变异株,设计了编码新冠病毒刺突蛋白(Spike)受体结构域(RBD)的环状 RNA 疫苗。 该项研究中制备的针对新冠病毒德尔塔变异株的环状 RNA 疫苗(circRNARBD
中科院高福院士课题组 2020 年发表了哪些重要的研究成果?
隆抗体 CB6 正式产品名为 JS016,目前已进入临床三期试验,前期数据表明能够有效治疗新冠轻中症感染患者。 图源:中科院微生物研究所 10、设计通用策略研发新冠病毒重组蛋白疫苗DOI: 10.1016/j.cell.2020.06.035疫苗可以说是解决新冠病毒蔓延最好的方式,新冠肺炎疫请发生后各国科学家都在争分夺秒地研究新冠疫苗,中国的疫苗研发也是一直处于世界前列。2020 年 8 月 6 日,高福院士联合严景华等在 Cell 杂志发文:A Universal Design
bat origin》中显示,2019 -nCoV 的全序列与一株来源于蝙蝠的 RaTG3 毒株相似度很高,达到 96.2 %。但随后有研究人员指出,2019 -nCoV 的 S 蛋白与蝙蝠冠状病毒 S 蛋白的差异较大(S -RBD 的序列相似度为 93.1 %),说明病毒直接从蝙蝠感染到人身上的难度很大,或许还存在一个或多个中间宿主。基于 2019 -nCoV WIV04 基因组全长序列的相似性比较图片来源:Nature华南农业大学的沈永义教授也认为,这一次新型冠状病毒的研究结果证明源头是蝙蝠
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