相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
SARS-CoV-2 (2019-nCoV) Spike RBD(Y453F)-His Recombinant Protein
- 保质期:
12个月
- 供应商:
北京义翘神州科技股份有限公司
- 保存条件:
-20℃ to -80℃
- 规格:
100 µg
纯度> 95 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Testing in progress
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(Y453F)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(Y453F)) was expressed with a polyhistidine tag at the C-terminus.
NP No.YP_009724390.1
表达宿主HEK293 Cells
种属2019-nCoV
预测 N 端Arg 319
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(Y453F)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.5 kDa. As a result of glycosylation, it migrates as an approximately 35.6 kDa band in SDS-PAGE under reducing conditions.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Figure eight (8字型):由尚未完成的重组生成的两个相互连接的环状DNA。 Filter hybridization (滤膜杂交):将变性DNA样本固定于硝酸纤维膜上,然后用放射性标记 的DNA 或RNA 进行杂交。 Fingerprint of DNA (DNA 指纹图谱):指不同基因组间的不同多形态限制性片段模式。 Fingerprint of protein (蛋白质指纹图谱):酶(如胰蛋白酶)切割蛋白质后产生的片段模式(通 常用双向凝胶电泳分离)。
,如 F 因子(F factor)(二)转化作用(transformation):通过自动获取或人为地供给外源DNA,使细胞或培养的受体细胞获得新的遗传表型。(三)转导作用(transduction):当病毒从被感染的(供体)细胞释放出来、再次感染另一(受体)细胞时,发生在供体细胞与受体细胞之间的DNA转移及基因重组即为转导作用。重组DNA技术一、重组DNA技术相关概念(一)DNA克隆(也称基因克隆或重组DNA)应用酶学的方法, 在体外将各种来源的遗传物质DNA与载体DNA接合成具有自我复制能力
中科院高福院士课题组 2020 年发表了哪些重要的研究成果?
动物)的 ACE2 可以与 SARS-CoV-2 受体结合域(RBD)并促进 SARS-CoV-2 假病毒的转导。与 SARS-CoV-2 相比,SARS-CoV 似乎在选择受体方面有稍宽的范围。研究进一步以 3Å 分辨率解析了猫 ACE2(cACE2)与 SARS-CoV-2 RBD 的复合体的低温电子显微镜(cryo-EM)结构,揭示了与 hACE2 类似的结合模式与 SARS-CoV- 2 RBD。这些结果为寻找 SARS-CoV-2 的中间宿主提供了亮点,并强调了监控易感宿主以防止进一步爆发的必要
技术资料暂无技术资料 索取技术资料









