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2019-nCoV Spike 重组蛋白|新冠病毒 Spike Protein (RBD, His Tag)

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  • ¥4500
  • 义翘神州
  • 北京
  • 2025年08月12日
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 库存

      999

    • 英文名

      SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag)

    • 保质期

      12个月

    • 供应商

      北京义翘神州科技股份有限公司

    • 保存条件

      -20℃ to -80℃

    • 规格

      100 µg

    SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag): 产品信息
    纯度:> 95 % as determined by SDS-PAGE
    内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
    生物活性:Measured by its binding ability in a functional ELISA.
    Immobilized human ACE2 protein (Fc tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag)(40592-V08H),the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag) is 500-900ng/mL.
    产品细节图片1
    蛋白构建:A DNA sequence encoding the NCP-CoV(2019-nCoV) Spike Protein (RBD) was expressed with a polyhistidine tag at the C-terminus.
    表达宿主:HEK293 Cells
    种属:2019-nCoV
    预测 N 端:Arg
    分子量:The recombinant NCP-CoV(2019-nCoV) Spike Protein (RBD, His Tag) consists of 234 amino acids and predicts a molecular mass of 23.54 kDa.  

    北京义翘神州科技股份有限公司(Sino Biological Inc.)创建于2007年,是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司,主要业务包括重组蛋白、抗体、基因和培养基等产品,以及重组蛋白、抗体的开发和生物分析检测等服务。

    产品细节图片2

    义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务,能够覆盖生命科学研究的多个领域,为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供“一站式”生物试剂产品和技术服务。客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲等地建立了子公司,在上海建立分公司,已成为生物试剂行业国内领先的科技公司之一。

    产品细节图片3

    凭借多年病毒蛋白研究技术优势,义翘神州仅用11天就成功表达出新冠病毒重要靶点受体结合域蛋白。为了更好的支持新冠病毒研究,义翘神州已开发生产近百种新冠病毒相关科研试剂,包括重要靶点抗原蛋白、中和抗体、抗原蛋白检测试剂盒等。义翘神州还可以提供新冠假病毒中和检测服务,可用于筛选出具有中和活性的抗体或血清,支持在细胞水平上的评估。目前,这些科研试剂已被全球数百家研究机构用于疫苗和抗体开发及基础机制研究。

    产品细节图片4

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    • 内容
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    图标文献和实验
    该产品被引用文献
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    4, Asandei A, et al. Non-Receptor-Mediated Lipid Membrane Permeabilization by the SARS-CoV-2 Spike Protein S1 Subunit.ACS applied materials & interfaces, PubMed ID: 33270413
    5, Pradenas E, et al. Stable neutralizing antibody levels 6 months after mild and severe COVID-19 episodes.Med (New York, N.Y.), PubMed ID: 33554155
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    8, Schasfoort RBM, et al. High throughput surface plasmon resonance imaging method for clinical detection of presence and strength of binding of IgM, IgG and IgA antibodies against SARS-CoV-2 during CoViD-19 infection.MethodsX, PubMed ID: 34221910
    9, Tang J, et al. Epitope diversity of SARS-CoV-2 hyperimmune intravenous human immunoglobulins and neutralization of variants of concern.iScience, PubMed ID: 34430803
    10, Wu K, et al. One-Step, Wash-free, Nanoparticle Clustering-Based Magnetic Particle Spectroscopy Bioassay Method for Detection of SARS-CoV-2 Spike and Nucleocapsid Proteins in the Liquid Phase.ACS applied materials & interfaces, PubMed ID: 34499464
    11, Maghsood F, et al. Differential Antibody Response to SARS-CoV-2 Antigens in Recovered and Deceased Iranian COVID-19 Patients.Viral immunology, PubMed ID: 34534012
    12, Egorov AI, et al. A Multiplex Noninvasive Salivary Antibody Assay for SARS-CoV-2 Infection and Its Application in a Population-Based Survey by Mail.Microbiology spectrum, PubMed ID: 34523986
    13, Wouters E, et al. A novel competition ELISA for the rapid quantification of SARS-CoV-2 neutralizing antibodies in convalescent plasma.Transfusion, PubMed ID: 34498761
    14, Tang J, et al. Antibody affinity maturation and cross-variant activity following SARS-CoV-2 mRNA vaccination: Impact of prior exposure and sex.EBioMedicine, PubMed ID: 34902788
    15, Qu JH, et al. Innovative FO-SPR Label-free Strategy for Detecting Anti-RBD Antibodies in COVID-19 Patient Serum and Whole Blood.ACS sensors, PubMed ID: 35061357
    16, Dutt TS, et al. Comprehensive Immune Profiling Reveals CD56+ Monocytes and CD31+ Endothelial Cells Are Increased in Severe COVID-19 Disease.Journal of immunology (Baltimore, Md. : 1950), PubMed ID: 34987111
    17, Fu Y, et al. Interfering effects on the bioactivities of several key proteins of COVID-19/variants in diabetes by compounds from Lianqiao leaves: In silico and in vitro analyses.International journal of biological macromolecules, PubMed ID: 35346677
    18, Lee Y, et al. SARS-CoV-2 mRNA vaccine induced higher antibody affinity and IgG titers against variants of concern in post-partum vs non-post-partum women.EBioMedicine, PubMed ID: 35301181
    19, Wussow F, et al. COH04S1 and Beta Sequence Modified Vaccine Protect Hamsters From SARS-CoV-2 Variants.iScience, PubMed ID: 35634578
    20, Bixler SL, et al. Exposure Route Influences Disease Severity in the COVID-19 Cynomolgus Macaque Model.Viruses, PubMed ID: 35632755
    21, Zimmerman O, et al. mRNA vaccine boosting enhances antibody responses against SARS-CoV-2 Omicron variant in individuals with antibody deficiency syndromes.Cell Reports Medicine, PubMed ID: 35688161
    22, Emmenegger M, et al. Both COVID-19 infection and vaccination induce high-affinity cross-clade responses to SARS-CoV-2 variants.iScience, PubMed ID: 35875683
    23, Wu F, et al. Single-shot AAV-vectored vaccine against SARS-CoV-2 with fast and long-lasting immunity.Acta pharmaceutica Sinica. B, PubMed ID: 35846427
    24, Chiuppesi F, et al. Vaccine-induced spike- and nucleocapsid-specific cellular responses maintain potent cross-reactivity to SARS-CoV-2 Delta and Omicron variants.iScience, PubMed ID: 35846380
    25, Besselink G, et al. Asymmetric Mach-Zehnder Interferometric Biosensing for Quantitative and Sensitive Multiplex Detection of Anti-SARS-CoV-2 Antibodies in Human Plasma.Biosensors, PubMed ID: 35892450
    26, Koklu A, et al. Convection Driven Ultrarapid Protein Detection via Nanobody-Functionalized Organic Electrochemical Transistors.Advanced materials (Deerfield Beach, Fla.), PubMed ID: 35772173
    27, Hawman DW, et al. Replicating RNA platform enables rapid response to the SARS-CoV-2 Omicron variant and elicits enhanced protection in naïve hamsters compared to ancestral vaccine.EBioMedicine, PubMed ID: 35932641
    28, Lu JG, et al. Structures, biomimetic synthesis, and anti-SARS-CoV-2 activity of two pairs of enantiomeric phenylpropanoid-conjugated protoberberine alkaloids from the rhizomes of Corydalis decumbens.Archives of pharmacal research, PubMed ID: 36121609
    29, Wu J, et al. CD147 contributes to SARS-CoV-2-induced pulmonary fibrosis.Signal transduction and targeted therapy, PubMed ID: 36424379
    30, Wu K, et al. Five-Minute Magnetic Nanoparticle Spectroscopy-Based Bioassay for Ultrafast Detection of SARS-CoV-2 Spike Protein.ACS applied nano materials, PubMed ID: 36570474
    31, Langel SN, et al. Dam-Infant Rhesus Macaque Pairs to Dissect Age-Dependent Responses to SARS-CoV-2 Infection.ImmunoHorizons, PubMed ID: 36547390
    32, Emmenegger M, et al. Protocol to determine antibody affinity and concentration in complex solutions using microfluidic antibody affinity profiling.STAR protocols, PubMed ID: 36853663
    33, Park KS, et al. Ultra-sensitive label-free SERS biosensor with high-throughput screened DNA aptamer for universal detection of SARS-CoV-2 variants from clinical samples.Biosensors & bioelectronics, PubMed ID: 36940632
    34, Adams LE, et al. Fc-mediated pan-sarbecovirus protection after alphavirus vector vaccination.Cell reports, PubMed ID: 37000623
    35, Tai W, et al. An mRNA-based T-cell-inducing antigen strengthens COVID-19 vaccine against SARS-CoV-2 variants.Nature communications, PubMed ID: 37221158
    36, Wouters E, et al. Intranasal administration of convalescent plasma protects against SARS-CoV-2 infection in hamsters.EBioMedicine, PubMed ID: 37148586
    37, Han X, et al. Adjuvant lipidoid-substituted lipid nanoparticles augment the immunogenicity of SARS-CoV-2 mRNA vaccines.Nature nanotechnology, PubMed ID: 37365276
    38, Chen X, et al. PD-1/PD-L1 blockade restores tumor-induced COVID-19 vaccine bluntness.Vaccine, PubMed ID: 37400286
    39, Tong X, et al. Waning and boosting of antibody Fc-effector functions upon SARS-CoV-2 vaccination.Nature communications, PubMed ID: 37443074
    40, Mulgaonkar N, et al. Montelukast and Telmisartan as Inhibitors of SARS-CoV-2 Omicron Variant.Pharmaceutics, PubMed ID: 37514075
    41, Ou BS, et al. Saponin Nanoparticle Adjuvants Incorporating Toll-Like Receptor Agonists Improve Vaccine Immunomodulation.bioRxiv : the preprint server for biology, PubMed ID: 37577608
    42, Chiuppesi F, et al. Stimulation of Potent Humoral and Cellular Immunity via Synthetic Dual-Antigen MVA-Based COVID-19 Vaccine COH04S1 in Cancer Patients Post Hematopoietic Cell Transplantation and Cellular Therapy.Vaccines, PubMed ID: 37766168
    43, Stewart-Jones GBE, et al. Domain-based mRNA vaccines encoding spike protein N-terminal and receptor binding domains confer protection against SARS-CoV-2.Science translational medicine, PubMed ID: 37703353
    44, Guo Y, et al. The cryo-EM structure of homotetrameric attachment glycoprotein from langya henipavirus.Nature communications, PubMed ID: 38280880
    45, Khan I, et al. Tubeimosides are pan-coronavirus and filovirus inhibitors that can block their fusion protein binding to Niemann-Pick C1.Nature communications, PubMed ID: 38167417
    46, Poulakou G, et al. Anti-SARS-CoV-2 glyco-humanized polyclonal antibody XAV-19: phase II/III randomized placebo-controlled trial shows acceleration to recovery for mild to moderate patients with COVID-19.Frontiers in immunology, PubMed ID: 38694503
    47, Kinoshita M, et al. Immunological Response to Subcutaneous and Intranasal Administration of SARS-CoV-2 Spike Protein in Mice.Vaccines, PubMed ID: 38675726
    48, Maji D, et al. Enhancing In Vivo Electroporation Efficiency through Hyaluronidase: Insights into Plasmid Distribution and Optimization Strategies.Pharmaceutics, PubMed ID: 38675208
    49, Park S, et al. An ancestral SARS-CoV-2 vaccine induces anti-Omicron variants antibodies by hypermutation.Nature communications, PubMed ID: 38643233
    50, Jansen EB, et al. PASC (Post Acute Sequelae of COVID-19) is associated with decreased neutralizing antibody titers in both biological sexes and increased ANG-2 and GM-CSF in females.Scientific reports, PubMed ID: 38684819
    51, Ou BS, et al. Saponin nanoparticle adjuvants incorporating Toll-like receptor agonists drive distinct immune signatures and potent vaccine responses.Science advances, PubMed ID: 39110802
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