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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
999
- 英文名:
SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag)
- 保质期:
12个月
- 供应商:
北京义翘神州科技股份有限公司
- 保存条件:
-20℃ to -80℃
- 规格:
100 µg
纯度:> 95 % as determined by SDS-PAGE
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
生物活性:Measured by its binding ability in a functional ELISA.
Immobilized human ACE2 protein (Fc tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag)(40592-V08H),the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag) is 500-900ng/mL.
蛋白构建:A DNA sequence encoding the NCP-CoV(2019-nCoV) Spike Protein (RBD) was expressed with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
种属:2019-nCoV
预测 N 端:Arg
分子量:The recombinant NCP-CoV(2019-nCoV) Spike Protein (RBD, His Tag) consists of 234 amino acids and predicts a molecular mass of 23.54 kDa.
北京义翘神州科技股份有限公司(Sino Biological Inc.)创建于2007年,是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司,主要业务包括重组蛋白、抗体、基因和培养基等产品,以及重组蛋白、抗体的开发和生物分析检测等服务。

义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务,能够覆盖生命科学研究的多个领域,为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供“一站式”生物试剂产品和技术服务。客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲等地建立了子公司,在上海建立分公司,已成为生物试剂行业国内领先的科技公司之一。

凭借多年病毒蛋白研究技术优势,义翘神州仅用11天就成功表达出新冠病毒重要靶点受体结合域蛋白。为了更好的支持新冠病毒研究,义翘神州已开发生产近百种新冠病毒相关科研试剂,包括重要靶点抗原蛋白、中和抗体、抗原蛋白检测试剂盒等。义翘神州还可以提供新冠假病毒中和检测服务,可用于筛选出具有中和活性的抗体或血清,支持在细胞水平上的评估。目前,这些科研试剂已被全球数百家研究机构用于疫苗和抗体开发及基础机制研究。
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- 作者
- 内容
- 询问日期
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样品:重组胰蛋白酶,比活 4500USP u/mg pro.,上海雅心生物技术有限公司,纯度如图 1 所示。BAEE,Sigma. 图 1. 重组胰蛋白酶的纯度的 SDS-PAGE 鉴定 1. 最适 pH 的研究 准备 pH 为 3~12 的底物 BAEE(0.25 mM),分别测定 rPT 纯酶液的活性,最后结果表示为测定数值占最高酶活的百分比。缓冲液成分依次为 25 mM NaAc-HAc (pH 3~6),25 mM Tris-HCl (pH 7~8),25
样品:重组胰蛋白酶,比活 4000USP u/mg pro.,上海雅心生物技术有限公司,纯度如图 1 所示。BAEE,Sigma. 图 1. 重组胰蛋白酶的纯度的 SDS-PAGE 鉴定 1. 最适温度的研究 将底物 BAEE(0.25 mM)分别置于 4℃、15℃、20℃、25℃、30℃、40℃、50℃、60℃ 和 70℃ 水浴中温浴 20 min,加入相同体积的 RPT 酶液测定 rPT 的活力,结果表示为测定数值占最高酶活的百分比。 2. 温度稳定性的研究 取
中科院高福院士课题组 2020 年发表了哪些重要的研究成果?
动物)的 ACE2 可以与 SARS-CoV-2 受体结合域(RBD)并促进 SARS-CoV-2 假病毒的转导。与 SARS-CoV-2 相比,SARS-CoV 似乎在选择受体方面有稍宽的范围。研究进一步以 3Å 分辨率解析了猫 ACE2(cACE2)与 SARS-CoV-2 RBD 的复合体的低温电子显微镜(cryo-EM)结构,揭示了与 hACE2 类似的结合模式与 SARS-CoV- 2 RBD。这些结果为寻找 SARS-CoV-2 的中间宿主提供了亮点,并强调了监控易感宿主以防止进一步爆发的必要










