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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海西宝
- 英文名:
14:0 PEG5000 PE
- 规格:
25mg
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文献和实验-forming compounds, micelles made of polyethylene glycol-phosphatidylethanolamine (PEG-PE) have gained more attention due to some attractive properties such as good stability, longevity, and ability to accumulate in the areas with an abnormal vasculature
liteng1117 求助: 构建好了重组腺病毒进行蛋白表达,分子量是14kd,5%的浓缩胶70v,12%,或15%的分离胶110v,跑90min。湿转:100v,90min。封闭用5%的脱脂奶粉,封闭3h(因为背景深,以为封闭久会好些)。一抗1:3000,4度过夜,二抗1:5000,孵育1h,洗膜都是用TBST漂洗15min ×4次。但是显影后背景特别深,虽然可以看见目的条带,但还是有一些非特异性的条带出现,不知道是封闭出问题还是一抗浓度过高。
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
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