EpiQuik Total Histone H3 Quant
ification Kit (Colorimetric) (96 assays), 总组蛋白H3定量试剂盒(比色法)(96次分析)- ￥6752
- Epigentek
- P-3062-96
- 美国
- 2025年09月03日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海西宝
- 英文名：
EpiQuik Total Histone H3 Quantification Kit (Colorimetric) (96 assays)
- 规格：
96次分析
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文献和实验1a).Part II Triple immunoprecipitation: H3K27 ChIP/-flag-DNMT3A/biotin-RNA A total of 4.0×106 293T cells are plated out and 24 hours later transfected plated (~70% confluent at the time of transfection) with the flag-tagged protein expressing plasmid
Whole genome amplification protocol for ChIP-chip
to a genomic tiling array. Therefore, we have adapted the standard protocol for whole genome amplification using the Sigma GenomePlex WGA kit to amplify our ChIP sample (O'Geen et al., 2006). Using Oct4 ChIP-chip assays as an example, we have compared
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
control) relative to the level of the signal of interest (ChIP sample). This hampers distinguishing a ''true signal'' from the background signal. Therefore, we recommend setting up ChIP using an antibody against an invariant domain of histone H3 [19,20
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