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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant full length protein of active Rab3A
- 亚型:
lgG
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
单克隆
- 标记物:
FITC
- 适应物种:
脊椎动物
- 保质期:
24个月
- 抗原来源:
蛋白表达
- 目录编号:
TD-26920
- 级别:
科研级
- 库存:
88
- 供应商:
武汉天德
- 宿主:
Mouse
- 应用范围:
IP, IHC, IF
- 浓度:
1mg/ml
- 靶点:
Rab3
- 抗体英文名:
Anti-Rab3-GTP Mouse Monoclonal Antibody
- 抗体名:
Rab3-GTP
- 规格:
30 μL
Rab3-GTP
| Cat. #: TD-26920 |
| Size: 30 µL |
| Gene Symbol: RAB3 |
| Description: Anti-Rab3-GTP Mouse Monoclonal Antibody |
| Background: Rab3 is a member of the Rab-family GTPases and is specifically targeted to synaptic vesicles. |
| Immunogen: Recombinant full length protein of active Rab3A |
| Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-Rab3-GTP monoclonal antibody recognizes active Rab3 proteins of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot: ![]() Rab3 activation assay. Purified full-length Rab3A proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-Rab3-GTP monoclonal antibody (Cat. No. TD-26920). Immunoblot was with an anti-Rab3A polyclonal antibody (Cat. No. ). |
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文献和实验Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases
, which undergo posttranslational modifications with geranylgeranyl moieties in most cases. Rab GTPases cycle between the guanosine diphosphate (GDP)-bound inactive and guanosine triphosphate (GTP)-bound active forms and between the cytosol and the membranes
小GTP结合蛋白(small GTP binding protein)
细胞中有两类结构不同的GTP结合蛋白:一种是单体GTP结合蛋白( 也称单体GTPase), 含有一条多肽链, 即是小GTP结合蛋白;另一种即是参与信号转导的三体G蛋白。 小GTP结合蛋白是细胞内的一个大的蛋白家族, 它以两种状态存在: 同GTP结合时, 具活性状态; 同GDP结合, 则是非活性状态。小GTP结合蛋白的活性和非活性状态的转变取决于两种蛋白: 一种是鸟嘌呤核苷释放蛋白(guanine-nucleotide-releasing protein, GNRP
GTP结合蛋白(GTP binding protein, G蛋白)
与GTP或GDP结合的蛋白质,又叫鸟苷酸结合调节蛋白(guanine nucleotide-binding regulatory protein)。从组成上看,有单体G蛋白(一条多肽链)和多亚基G蛋白(多条多肽链组成)。G蛋白参与细胞的多种生命活动,如细胞通讯、核糖体与内质网的结合、小泡运输、蛋白质合成等。 G蛋白偶联系统中的G蛋白是由三个不同亚基组成的异源三体,三个亚基分别是α、β、γ, 总相对分子质量在100kDa左右, β亚基为36 kDa左右, γ亚基
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