IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
浓度:
1mg/ml
靶点:
Rab4
抗体英文名:
Anti-Rab4-GTP Monoclonal Antibody
抗体名:
Rab4-GTP
规格:
30 μL
Rab4-GTP
Cat. #: TD-26921
Size: 30 µL
Gene Symbol: RAB4
Description: Anti-Rab4-GTP Monoclonal Antibody
Background: Rab-4 belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20, RUFY1, SGSM1, SGSM2 and SGSM3. It participates in protein transport and in vesicular traffic.
Immunogen: Recombinant full length protein of active Rab4A.
Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration: 1 mg/ml
Host Species: Mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Preservative: No
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-Rab4-GTP monoclonal antibody recognizes active Rab4 from vertebrates.
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:
Rab4A Activation Assay. Purified GST-tagged Rab4A proteins (Cat. #10130) were immunoprecipitated with the anti-Rab4-GTP monoclonal antibody (Cat. No. TD-26921) after treated with GDP (lane 1) or GTPγ S (lane 2), and was blotted with anti-Rab4A polyclonal antibody (Cat. No. ). Input control is shown in the bottom panel.