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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉天德
- 库存:
999
- 级别:
科研级
- 抗体英文名:
Anti-Swine IgM mouse monoclonal antibody
- 抗体名:
Swine IgM
- 规格:
25 μl/100 μl/200 μl
Anti-Swine IgM mouse monoclonal antibody
| Product Name | Anti-Swine IgM mouse monoclonal antibody |
| Cat.# | TD-AB0100 |
| Package | 25 μl/100 μl/200 μl |
| Storage | PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 |
| Product Imformation | |
| Description | |
| Applications | WB |
| Immunogen | Swine |
| Reactivity | Swine IgM |
| Host species | Mouse |
| Ig class | IgG |
| Purity | Protein A/G Affinity Purification |
| Target information | |
| Symbol | Swine IgM |
| Synonyms | |
| Target Background | |
| IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. | |
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文献和实验Production of Monoclonal Antibodies in Swine
A major objective of this laboratory has been to improve the growth performance of swine with immunological manipulation. During the progress of the program, we demonstrated that a mouse monoclonal antibody (MAb) was able to enhance
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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