- ￥4800
- Tanda
- 美国
- TD-26908
- 2025年10月20日
- IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant full length protein of active Gαz
- 亚型：
lgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白表达
- 目录编号：
TD-26908
- 级别：
科研级
- 库存：
88
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
- 浓度：
1mg/ml
- 靶点：
Gαz
- 抗体英文名：
Anti-Gαz-GTP Mouse Monoclonal Antibody
- 抗体名：
Gαz-GTP
- 规格：
30 μL
Gαz-GTP
| Cat. #: TD-26908 |
| Size: 30 µL |
| Gene Symbol: Gnaz |
| Description: Anti-Gαz-GTP Mouse Monoclonal Antibody |
| Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαz represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαz proteins are being explored. |
| Immunogen: Recombinant full length protein of active Gαz |
| Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-Gαz-GTP monoclonal antibody recognizes active Gαz of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot:  Gαz activation assay. Purified Gαz proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-Gαz-GTP monoclonal antibody (Cat. No. TD-26908). Immunoblot was with an anti-Gαz polyclonal antibody. |
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文献和实验On activation, G-protein-coupled receptors (GPCRs) exert many of their cellular actions through promotion of guanine nucleotide exchange on the Gα-subunit of heterotrimeric G-proteins to release free Gα-GTP and βγ-subunits. In membrane
Measurement of GTP-Binding and GTPase Activity of Heterotrimeric G Proteins
activities of Gα protein, which binds and hydrolyses GTP. Evaluation of the rate of GTP binding, the rate of GTP hydrolysis, and the rate of GTP/GDP exchange on Gα protein are required to better understand the mechanistic aspects of heterotrimeric G-protein
Identification and Quantitation of G-Protein α-Subunits
The demonstration that many intracellular signaling processes are mediated by a family of closely related guanine nucleotide binding proteins (G-proteins) has led to the development of specific techniques that can be used to identify
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