Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαz represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαz proteins are being explored.
Immunogen: Recombinant full length protein of active Gαz
Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration: 1 mg/ml
Host Species: Mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Preservative: No
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-Gαz-GTP monoclonal antibody recognizes active Gαz of vertebrates.
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:
Gαz activation assay. Purified Gαz proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-Gαz-GTP monoclonal antibody (Cat. No. TD-26908). Immunoblot was with an anti-Gαz polyclonal antibody.