PIK3CA (E542Q)产品图

PIK3CA (E542Q)

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  • ¥4800
  • Tanda
  • 美国
  • TD-26210
  • 2026年08月19日
  • ELISA, WB, IHC
  • Mouse
  • 脊椎动物
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原:

      A synthetic peptide from the internal region of PIK3CA which includes the mutation of E542Q, human origin.

    • 亚型:

      lgG

    • 形态:

      液体

    • 保存条件:

      -20℃

    • 克隆性:

      单克隆

    • 标记物:

      FITC

    • 适应物种:

      脊椎动物

    • 保质期:

      24个月

    • 抗原来源:

      蛋白

    • 目录编号:

      TD-26210

    • 级别:

      科研级

    • 库存:

      999

    • 供应商:

      武汉天德

    • 宿主:

      Mouse

    • 应用范围:

      ELISA, WB, IHC

    • 浓度:

      1mg/ml

    • 靶点:

      E542Q

    • 抗体英文名:

      Anti PIK3CA(E542Q) Mouse Monoclonal Antibody

    • 抗体名:

      PIK3CA(E542Q)

    • 规格:

      100 μL

    PIK3CA(E542Q)

    Cat. #:  TD-26210
    Size:  100 µL
    Gene Symbol:  PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
    Description:   Anti-PIK3CA(E542Q) Mouse Monoclonal Antibody
    Background:   Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
    Immunogen:   A synthetic peptide from the internal region of PIK3CA which includes the mutation of E542Q, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host Species:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Preservative: No
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes E542Q mutant, but not wild type PIK3CA of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
    Western blot: 
     产品细节图片1
    Western blot analysis of recombinant PIK3CA(E542Q) and wild type proteins. Purified His-tagged PIK3CA(E542Q) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-PIK3CA(E542Q) monoclonal antibody (Cat. #TD-26210).
    Immunofluorescence: 
     产品细节图片2
    Immunofluorescence of cells expressing PIK3CA proteins with Anti-PIK3CA(E542Q) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA(E542Q) plasmid (right column), then fixed and stained with Anti-PIK3CA(E542Q) monoclonal antibody (Cat. #TD-26210).



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    图标文献和实验
    相关实验
    • 在Matlab中探索基因表达数据分析

      q-values PAFAH1B3 10.211 8.1924e-009 1.8315e-005 1.8315e-005 RAB31 -13.702 2.8016e-008 3.1316e-005 3.1316e-005 LRP1 -9.0742 1.6453e-007 0.00012261 5.3953e-005 KIAA0367 -8.9398 1.7404e-007 9.7272e-005 5.3953e-005 ID2B

    • Cystic Fibrosis

      and frequency of mutations is very variable from ethnic population to population (4 ). The most common mutation, F508del (e.g., ΔF508 according to the old nomenclature), reaches frequencies of about 70% in Northern European populations. Besides F508del

    • Adv Sci∣周祥团队合作揭示通过工程化外泌体诱导肿瘤细胞核仁应激的分子机制与策略

      研究员,南昌大学基础医学院陈加祥教授和南昌大学第一附属医院邓军主任医师为论文的共同通讯作者。复旦大学附属肿瘤医院甘雨博士、郝茜博士和新乡医学院健康中原研究院韩涛教授为论文共同第一作者。该工作还得到了宇玫博生物的大力支持。   文献原文: 【1】Gan Y, Hao Q, Han T, Tong J, Yan Q, Zhong H, Gao B, Li Y, Xuan Z, Li P, Yao L, Xu Y, Jiang YZ, Shao ZM, Deng J, Chen J, Zhou X

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