PIK3CA(E542Q)

PIK3CA(E542Q)

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  • ¥4800
  • Tanda
  • 美国
  • TD-26210
  • 2025年12月10日
  • ELISA, WB, IHC
  • Mouse
  • 脊椎动物
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    • 详细信息
    • 技术资料
    • 免疫原

      A synthetic peptide from the internal region of PIK3CA which includes the mutation of E542Q, human origin.

    • 亚型

      IgG

    • 形态

      液体

    • 保存条件

      -20℃

    • 克隆性

      单克隆

    • 标记物

      FITC

    • 适应物种

      脊椎动物

    • 保质期

      24个月

    • 抗原来源

      蛋白

    • 目录编号

      TD-26210

    • 级别

      科研级

    • 库存

      999

    • 供应商

      武汉天德

    • 宿主

      Mouse

    • 应用范围

      ELISA, WB, IHC

    • 浓度

      1mg/ml

    • 靶点

      E542Q

    • 抗体英文名

      Anti PIK3CA(E542Q) Mouse Monoclonal Antibody

    • 抗体名

      PIK3CA(E542Q)

    • 规格

      100μl

    PIK3CA(E542Q)

    Cat. #:  TD-26210
    Size:  100 µL
    Gene Symbol:  PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
    Description:   Anti-PIK3CA(E542Q) Mouse Monoclonal Antibody
    Background:   Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
    Immunogen:   A synthetic peptide from the internal region of PIK3CA which includes the mutation of E542Q, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host Species:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Preservative: No
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes E542Q mutant, but not wild type PIK3CA of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
    Western blot: 
     PIK3CA(E542Q)
    Western blot analysis of recombinant PIK3CA(E542Q) and wild type proteins. Purified His-tagged PIK3CA(E542Q) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-PIK3CA(E542Q) monoclonal antibody (Cat. #TD-26210).
    Immunofluorescence: 
     PIK3CA(E542Q)
    Immunofluorescence of cells expressing PIK3CA proteins with Anti-PIK3CA(E542Q) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA(E542Q) plasmid (right column), then fixed and stained with Anti-PIK3CA(E542Q) monoclonal antibody (Cat. #TD-26210).

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