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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide surround the 777A codon of ErbB2 protein.
- 亚型:
lgG
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
单克隆
- 标记物:
FITC
- 适应物种:
脊椎动物
- 保质期:
24个月
- 抗原来源:
蛋白
- 目录编号:
TD-26080
- 级别:
科研级
- 库存:
999
- 供应商:
武汉天德
- 宿主:
Mouse
- 应用范围:
ELISA, WB, IF, IHC
- 浓度:
0.2mg/ml
- 靶点:
V777A
- 抗体英文名:
Anti ErbB2(V777A) mouse monoclonal Antibody
- 抗体名:
ErbB2(V777A)
- 规格:
100 μL
ErbB2(V777A)
| Cat. #: TD-26080 |
| Gene Symbol: ERBB2/HER2/Neu |
| Description: Anti-ErbB2(V777A) Mouse Monoclonal Antibody |
| Background: The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis. Mutations of ErbB2 protein, including V777A, have been indicated in human cancers. |
| Immunogen: A synthetic peptide surround the 777A codon of ErbB2 protein. |
| Tested Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:500-1:5000 WB: 1:200-1:2000 IF: 1:100 IHC: 1:50-1:100 |
| Concentration: 0.2 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes V777A mutant but not wild type ErbB2 protein, of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. |
Immunofluorescence:
|
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文献和实验of E-cadherin mRNA is decreased, especially with use of several siRNAs. Furthermore, concentrations of Ecadherin decrease in parallel with the mRNA. The authors suggest that this siRNA-induced methylation was mediated by DNA methylases 1 and 3b. ERBB2
Her2为啥是ErbB信号通路的关键呢?因为没有她,就没法穿搭……
ErbB信号通路,是个做妇科肿瘤常见的信号通路,特别是乳腺癌研究中,对HER2的研究都很常见。为啥HER2(也就是ErbB2)的研究会这么多,这和ErbB信号通路本身是分不开的。我们先来看看ErbB信号通路是什么鬼?嗯,这个复杂程度,就有点令人发指了,不光是进入细胞后的内容丰富,膜受体也是特别复杂。夏老师给你们稍微简化一下,差不多就是这样:还是有点复杂,不是么?实际上,造成ErbB信号通路这么复杂的原因,主要就是ErbB信号通路的膜受体,一共有这么四种:ErbB1(也就是EGFR),ErbB2
,为了实现基因靶向转移,可以构建一种可溶性分子复合物载体系统,它包含两个功能区:DNA结合区,常用L―多聚赖氨酸、脂质体等,它们通过静电方式结合DNA或将DNA包裹;配体或抗体区,它们通过识别肿瘤细胞表面受体或抗原,选择性地内化进入肿瘤细胞。Uherek等将酵母转录因子Gal4的DNA结合结构域与白喉毒素转膜结构域、抗ErbB2抗体片段依次连接,构建了一种新的融合蛋白载体,它可与质粒形成复合物,用L―多聚赖氨酸处理后,可特异性地将外源基因导人ErbB2阳性的肿瘤细胞中。
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