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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide from the internal region of HRas which includes the mutation of G13A, human origin.Peptide
- 亚型:
lgG
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
单克隆
- 标记物:
FITC
- 适应物种:
脊椎动物
- 保质期:
24个月
- 抗原来源:
蛋白
- 目录编号:
TD-26191
- 级别:
科研级
- 库存:
999
- 供应商:
武汉天德
- 宿主:
Mouse
- 应用范围:
ELISA, WB, IHC
- 浓度:
1mg/ml
- 靶点:
G13A
- 抗体英文名:
Anti HRAS(G13A) Mouse Monoclonal Antibody
- 抗体名:
Ras(G13A)
- 规格:
100 μL
Ras(G13A)
| Cat. #: TD-26191 |
| Gene Symbol: H-Ras; K-Ras; N-Ras |
| Description: Anti-HRAS(G13A) Mouse Monoclonal Antibody |
| Background: Belongs to the small GTPase superfamily. Ras family binds GDP/GTP and possess intrinsic GTPase activity, alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).Located in cell membrane, lipid-anchor, cytoplasmic side and Golgi apparatus membrane. |
| Immunogen: A synthetic peptide from the internal region of HRas which includes the mutation of G13A, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes G13A mutant, but not wild type H-RAS of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
|
Western blot:
Immunofluorescence:
|
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文献和实验Ras是大鼠肉瘤(rat sarcoma,Ras)的英文缩写。Ras蛋白是原癌基因 c—ras的表达产物,相对分子质量为21kDa,属单体 GTP结合蛋白,具有弱的 GTP酶活性。Ras蛋白的活性状态对细胞的生长、分化、细胞骨架、蛋白质运输和分泌等都具有影响,其活性则是通过与GTP或GDP的结合进行调节。 Ras的活性受两个蛋白的控制,一个是鸟苷交换因子(guanine nucleotide exchange factor, GEF),它的作用是促使GDP从Ras蛋白
Mathematical Investigation of How Oncogenic Ras Mutants Promote Ras Signaling
We have used a mathematical model of the Ras signaling network to link observable biochemical properties with cellular levels of RasGTP. Although there is abundant data characterizing Ras biochemistry, attributing specific changes
PNAS:RAS 抑制剂又添一员,新型蛋白模拟物或为癌症治疗
导读RAS 是肿瘤患者中最常见的致癌基因之一,目前包括 KRAS、NRAS 以及 HRAS 三种亚型。其中,NRAS 突变多见于黑色素瘤和急性髓系白血病,HRAS 突变多见于膀胱癌和头颈癌,而 KRAS 突变比其他两种更常见,最常发生在肺癌、胰腺癌以及结直肠癌。由于在癌症中的 Ras 蛋白经常发生突变,因此 Ras 突变成为了科学家们着力攻克的热点。RAS 曾被医学界称为「不可成药靶点」,科学家们在研究有效的 RAS 抑制剂方面已经摸索尝试了 30 余年,直至近几年才获得了重大进展。当前已经
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