Background: Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, recombination and regulation of chromatin architecture.
Immunogen: A synthetic peptide from the internal region of CTCF which includes the mutation of K344E, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes K344E mutant, but not wild type CTCF of vertebrates.
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing
Western blot:
Western blot analysis of recombinant CTCF (K344E) and wild type proteins.
Purified His-tagged CTCF (K344E) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-CTCF(K344E) monoclonal antibody (Cat. #TD-26324).
Immunofluorescence:
Immunofluorescence of cells expressing CTCF proteins with Anti-CTCF(K344E) antibody.
HEK293T cells were transfected with pCDNA3-GFP-CTCF (WT) plasmid (left column) or pCDNA3-GFP-CTCF (K344E) plasmid (right column), then fixed and stained with Anti-CTCF(K344E) monoclonal antibody (Cat. #TD-26324).