- ￥4800
- Tanda
- 美国
- TD-26082
- 2025年10月29日
- ELISA, WB, IF, IHC
- Mouse
- 脊椎动物
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- 详细信息
- 技术资料
- 免疫原：
A synthetic peptide from the internal region of EGFR which includes the mutation of L858R, human origin.
- 亚型：
IgG1
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白
- 目录编号：
TD-26082
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
ELISA, WB, IF, IHC
- 浓度：
1mg/ml
- 靶点：
L858R
- 抗体英文名：
Anti EGFR(L858R) Mouse Monoclonal Antibody
- 抗体名：
EGFR(L858R)
- 规格：
100 μL
EGFR(L858R)
| Cat. #: TD-26082 |
| Gene Symbol: EGFR/HER1/ERBB1 |
| Description: Anti-EGFR(L858R) Mouse Monoclonal Antibody |
| Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). |
| Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of L858R, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG1 |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes EGFR(L858R), but not wild type EGFR from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. |
Western blot:
Immunofluorescence:
Immunofluorescence of cells expressing EGFR proteins with anti EGFR(L858R) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR L858R) plasmid (right column), then fixed and stained with anti- EGFR(L858R) monoclonal antibody (Cat. # TD-26082).
Immunohistochemistry:
Immunohistochemical analysis of paraf\fin-embedded human NSCLC tissue with anti-EGFR(L858R) monoclonal antibody (Cat. #TD-26082). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:400) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:100) was used as secondary antibody.
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