AKT1 Polyclonal Antibody

AKT1 Polyclonal Antibody

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  • ¥2600
  • Tanda
  • 美国
  • TD-21274
  • 2025年10月24日
  • ELISA, WB, IHC
  • 脊椎动物
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    • 详细信息
    • 技术资料
    • 免疫原

      Purified AKT1 protein, human origin.

    • 亚型

      lgG

    • 形态

      液体

    • 保存条件

      -20℃

    • 克隆性

      多克隆

    • 标记物

      详询

    • 适应物种

      脊椎动物

    • 保质期

      24个月

    • 抗原来源

      蛋白

    • 目录编号

      TD-21274

    • 级别

      科研级

    • 库存

      999

    • 供应商

      武汉天德

    • 宿主

    • 应用范围

      ELISA, WB, IHC

    • 浓度

      0.2mg/ml

    • 靶点

      AKT1

    • 抗体英文名

      Anti-AKT1 Rabbit polyclonal antibody

    • 抗体名

      Tanda

    • 规格

      100 μL

    AKT1 Polyclonal Antibody

    Cat. #:  TD-21274
    Gene Symbol:  AKT1, AKT, PKB, PKB ALPHA, PRKBA, RAC, RAC ALPHA
    Description:   Anti-AKT1 Rabbit Polyclonal Antibody
    Background:   AKT1 is one of 3 closely related serine/threonine protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers.
    Immunogen:   Purified AKT1 protein, human origin.
    Applications:    ELISA, WB, IHC
    Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:200
    Concentration:   0.2mg/ml
    Host Species:   Rabbit
    Format:   Liquid
    Clonality:   Polyclonal
    Isotype:   IgG
    Purity:   Purified from serum
    Preservative: No
    Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes AKT1 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
    Western blot: 

     AKT1 Polyclonal Antibody
    Western blot analysis of recombinant AKT1 protein. Purified His-tagged AKT1 protein was blotted with Anti-AKT1 rabbit polyclonal antibody (Cat. #TD-21274). 
    Immunohistochemistry: 

     AKT1 Polyclonal Antibody
    Immunohistochemical analysis of paraf\fin-embedded human thyroid cancer tissue with Anti-AKT1 polyclonal antibody (Cat. #TD-21274). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.

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