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β-Catenin Polyclonal Antibody

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  • ¥2600
  • Tanda
  • 美国
  • TD-21282
  • 2025年10月24日
  • ELISA, WB, IF, IHC
  • 脊椎动物
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      A synthetic peptide from the internal region of β-catenin.

    • 亚型

      lgG

    • 形态

      液体

    • 保存条件

      -20℃

    • 克隆性

      多克隆

    • 标记物

      FITC

    • 适应物种

      脊椎动物

    • 保质期

      24个月

    • 抗原来源

      蛋白

    • 目录编号

      TD21282

    • 级别

      科研级

    • 库存

      999

    • 供应商

      武汉天德

    • 宿主

    • 应用范围

      ELISA, WB, IF, IHC

    • 浓度

      1mg/ml

    • 靶点

      CTNNB1

    • 抗体英文名

      Anti-CTNNB1 Rabbit polyclonal antibody

    • 抗体名

      β-Catenin Polyclonal Antibody

    • 规格

      100 μL

    β-Catenin Polyclonal Antibody

    Cat. #:  TD-21282
    Gene Symbol:  CTNNB1, CTNNB
    Description:   Anti-β-catenin Rabbit Polyclonal Antibody
    Background:   Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.
    Immunogen:    A synthetic peptide from the internal region of β-catenin.
    Applications:   ELISA, WB, IF, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000
    WB: 1:500-1:2000
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host Species:   Rabbit
    Format:   Liquid
    Clonality:   Polyclonal
    Isotype:   IgG
    Purity:    Purified from serum
    Preservative: No
    Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes β-catenin from vertebrates.
    Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
    Western blot:  
    产品细节图片1
    Western blot analysis of recombinant β-catenin protein. Purified His-tagged β-catenin protein (amino acids 1-76) was blotted with anti- β-catenin rabbit polyclonal antibody (Cat. #TD-21282).
    Immunohistochemistry:  
    产品细节图片2
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-β-catenin polyclonal antibody (Cat. #TD-21282). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.



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    图标文献和实验
    相关实验
    • Polyclonal Antibody Production

      , if desired this is best accomplished by Protein A Affinity Chromatography.   上一篇:make an Antibody against a Peptides   下一篇:Immunization Protocol for Monoclonal Antibody Production

    • Polyclonal Antibody Production

        Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.     Rabbit Immunization Initial Preparation Your antigen

    • Construction of Polyclonal Antibody Libraries Using Phage Display

      Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified

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