Flow cytometric analysis of lysed blood from a healthy donor. First, debris, doublets, and dead cells were excluded from the analysis (A−D). Cells were also gated based on CD14 and CD20 expression Ɛ. From CD14/CD20 negative population, different DC cell subsets, pDC, cDC1 and cDC2 were assessed (F−I) by measurement of CD123, BDCA-2, BDCA-3, CD1c, and FCR1 protein expression.