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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
Rod Clamp for 1.5 inch diameter rod Newport 340-RC 2 Rod, 14 inch (35.5 cm) tall Newport
Use of HMS Genetics Confocal Microscope
in the back on the upper right. • 40X and 100X objectives are oil imersion. • Filter 3 is red, Filter 2 is red+green, Filter 4 is confocal. • NOTE: for confocal scans the rod on the upper left of the eyepiece must be pulled out. trial scan • Set
BrdU-Immunoperoxidase Staining of Paraffin Tissue Sections
: Dilute (10x stock, -20ºC) 1/5 in PBS 2.5 N HCl: Dilute concentrated HCl 1/22 (v/v) in H2 O Mouse anti-BrDU: DAKO cat#M0744, $205/mL Biotinylated horse anti-mouse IgG, Avidin, biotin-HRP, Horse serum: Vector, Vectastain ABC kit, #PK4002 $185 Color
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