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- 9051
- 2025年07月12日
- Clinical Lab
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Clinical Lab
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Minicon® and Miniplus® ConcentratorsConcentrate urine and cerebrospinal fluid to intensify proteins that indicate abnormal or pathological states prior to analysis by electrophoresis or immunoelectrophoresis (e.g., Bence Jones proteins in urine). |
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文献和实验TOP10 chemically competent cells
reduces transformation efficiency by about 3x the first time, and about 6x total after several freeze/thaw cycles. Measurement of competence Transform 50 μl of cells with 1 μl of standard pUC19 plasmid (Invitrogen) This is at 10 pg/μl
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
2. Table top centrifuge capable at least 13,000 x g 3. 1.5 or 2 ml Nuclease-Free centrifuge tubes 4. 0.45 μm filter unit 实验步骤 Before starting: 1. Fresh or flash-frozen cultured cells can be used
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
by inverting the tube a few times. Incubate 5 minutes at room temperature. Invert the tube several times during incubation. NOTE: ERL Buffer is supplied as a 10x concentrate and must be diluted with ddH2 O according to bottle label. 2. Centrifuge
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