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    研选β-肌动蛋白/β-Actin 抗体(内参抗体)

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    β-肌动蛋白/β-Actin 抗体(内参抗体)

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    • 北京市通州区马驹桥镇联东U谷西区二号院67号楼
      • 货号:

        bs-0061R

      • 抗体名:

        β-肌动蛋白/β-Actin 抗体(内参抗体)

      • 抗体英文名:

        FITCbeta-Actin (Loading Control)

      • 靶点:

        beta-Actin (Loading Control)

      • 浓度:

        1mg/ml

      • 应用范围:

        WB:1:5000-20000 IHC-P:1:500-1000 Flow-Cyt:1μg/Test ELISA:1:5000-20000

      • 宿主:

        Rabbit

      • 供应商:

        北京博奥森生物技术有限公司

      • 数量:

        100

      • 级别:

        生产厂家

      • 目录编号:

        bs-0061R

      • 抗原来源:

        Synthetic MAP peptide derived from human beta-Actin

      • 保质期:

        一年

      • 适应物种:

        Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Rabbit,Sheep, Bee, Fish,GuineaPig, Hamster, Goat, Cat, mt,op

      • 标记物:

      • 克隆性:

        多克隆

      • 保存条件:

        Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

      • 形态:

        Lyophilized or Liquid

      • 亚型:

        IgG

      • 免疫原:

        Synthetic MAP peptide derived from human beta-Actin

      • 规格:

        20ul

      规格 价格
      20ul 480元
      50ul 680元
      100ul 980元
      200ul 1780元

      产品优势:Bioss金牌产品beta-Actin内参抗体270+篇文献引用,IF 800+
      1.交叉反应丰富:
      Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Bee, Fish, Guinea Pig, Hamster, Goat, Cat, mt,op
      2.特异性强;
      3.稀释比例适中:WB=1:5000-20000 ELISA=1:5000-20000 IHC-P=1:500-1000 Flow-Cyt=1μg/Test 

       
      产品编号 bs-0061R
      英文名称 beta-Actin (Loading Control)
      中文名称 β-肌动蛋白/β-Actin 抗体(内参抗体)
      产品类型 内参抗体 
      研究领域 肿瘤  细胞生物  信号转导  细胞骨架  
      抗体来源 Rabbit
      克隆类型 Polyclonal
      交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Bee, Fish, Guinea Pig, Hamster, Goat, Cat, mt,op
      产品应用 WB=1:5000-20000 ELISA=1:5000-20000 IHC-P=1:500-1000 Flow-Cyt=1μg/Test (石蜡切片需做抗原修复) 
      not yet tested in other applications.
      optimal dilutions/concentrations should be determined by the end user.
      分 子 量 42kDa
      细胞定位 细胞浆 
      性    状 Lyophilized or Liquid
      浓    度 1mg/ml
      免 疫 原 Synthetic MAP peptide derived from human beta-Actin:1-200/375 
      亚    型 IgG
      纯化方法 affinity purified by Protein A
      储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
      保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
      PubMed PubMed
      产品介绍 Loading Control
      This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008].

      Function:
      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

      Subunit:
      Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2.

      Subcellular Location:
      Cytoplasm. cytoskeleton.

      Tissue Specificity:
      Ubiquitously expressed in all eukaryotic cells.

      Post-translational modifications:
      ISGylated.
      Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.

      DISEASE:
      Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.

      Similarity:
      Belongs to the actin family.

      SWISS:
      P60709

      Gene ID:
      60

      Database links:
      Entrez Gene: 396526 Chicken
      Entrez Gene: 60 Human
      Entrez Gene: 11461 Mouse
      Entrez Gene: 100009272 Rabbit
      Entrez Gene: 81822 Rat
      Omim: 102630 Human
      SwissProt: P60706 Chicken
      SwissProt: P60712 Cow
      SwissProt: P60708 Horse
      SwissProt: P60709 Human
      SwissProt: P60710 Mouse
      SwissProt: P29751 Rabbit
      SwissProt: P60711 Rat
      SwissProt: P60713 Sheep
      Unigene: 520640 Human
      Unigene: 708120 Human
      Unigene: 727576 Human
      Unigene: 328431 Mouse
      Unigene: 391967 Mouse
      Unigene: 94978 Rat


      Important Note:
      This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

      内参抗体
      β-Actin是横纹肌肌纤维中的一种主要蛋白质成分,也是肌肉细丝及细胞骨架微丝的主要成分。具有收缩功能,分布广泛,具有高度保守性,在细胞中的表达相对稳定,因此常被用作校正系统的内参。β-Actin分子量为42 kDa,
      此抗体主要用于标记平滑肌及其来源的肿瘤。
      我公司开发的β-Actin抗体已被国内外广大科研工作者使用,被称谓:质量信得过产品.



       
      产品图片
      Sample: 
      Lung (Mouse) Lysate at 40 ug
      Uterus (Mouse) Lysate at 40 ug
      Small intestine (Mouse) Lysate at 40 ug
      Primary: Anti- beta-Actin (bs-0061R) at 1/2000 dilution
      Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
      Predicted band size: 42 kD
      Observed band size: 42 kD
      Sample: 
      A549 Cell (Human) Lysate at 30 ug
      Primary: Lane1: Anti-beta-Actin (bs-0061R) at 1/500 dilution
      Lane2: Anti-beta-Actin (bs-0061R) at 1/1000 dilution
      Lane3: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
      Lane4: Anti-beta-Actin (bs-0061R) at 1/4000 dilution
      Lane5: Anti-beta-Actin (bs-0061R) at 1/5000 dilution
      Lane6: Anti-beta-Actin (bs-0061R) at 1/8000 dilution
      Lane7: Anti-beta-Actin (bs-0061R) at 1/10000 dilution
      Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
      Predicted band size: 42 kD
      Observed band size: 42 kD
      Sample: 
      A549 Cell (Human) Lysate at 30 ug
      Primary: Lane1: Anti-beta-Actin (bs-0061R) at 1/500 dilution
      Lane2: Anti-beta-Actin (bs-0061R) at 1/1000 dilution
      Lane3: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
      Lane4: Anti-beta-Actin (bs-0061R) at 1/4000 dilution
      Lane5: Anti-beta-Actin (bs-0061R) at 1/5000 dilution
      Lane6: Anti-beta-Actin (bs-0061R) at 1/8000 dilution
      Lane7: Anti-beta-Actin (bs-0061R) at 1/10000 dilution
      Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
      Predicted band size: 42 kD
      Observed band size: 42 kD
      Sample: 
      Lane1: 293T Cell Lysate at 25 ug
      Lane2: A549 Cell Lysate at 25 ug
      Lane3: A431 Cell Lysate at 25 ug
      Primary: Anti- beta-Actin (bs-0061R) at 1/1000 and 1/5000 dilution
      Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
      Predicted band size: 42kD
      Observed band size: 42 kD
      Sample: Lung lysate at 30ug;
      Primary: Anti-beta-actin (bs-0061R) at 1:1000 dilution 
      Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1:3000 dilution
      Predicted band size : 42kD
      Observed band size : 42kD
      Sample: 
      Lung (Mouse) Lysate at 40 ug
      Cerebrum (Mouse) Lysate at 40 ug
      Primary: Anti-beta-Actin (Loading Control) (bs-0061R) at 1/2000 dilution
      Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
      Predicted band size: 42 kD
      Observed band size: 42 kD
      Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
      Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
      Incubation: Anti-Beta-actin Polyclonal Antibody, Unconjugated(bs-0061R) 1:1500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
      Blank control: RSC96(blue). 
      Primary Antibody: Rabbit Anti-beta-Actin /FITC Conjugated antibody (bs-0061R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; 
      Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
      Protocol
      The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-0061R/FITC, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
      别    名 Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin;A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN.  
       
      Specific References  (188)     |     bs-0061R has been referenced in 188 publications.
      [IF=6.36] Yoshino, Hirofumi, et al. "microRNA-210-3p depletion by CRISPR/Cas9 promoted tumorigenesis through revival of TWIST1 in renal cell carcinoma." Oncotarget.   WB ;  Human.  
      [IF=3.39] Li, Yanhui, et al. "Role of the Lateral Habenula in Pain-Associated Depression." Frontiers in Behavioral Neuroscience 11 (2017): 31.  WB ;  Rat.  
      [IF=1.84] Yao, Xiaolei, et al. "Characterization of GALNTL5 gene sequence and expression in ovine testes and sperm." Theriogenology (2017).  WB ;  Sheep.  
      [IF=2.25] Xiong, Zhang, et al. "Curcumin mediates presenilin-1 activity to reduce β-amyloid production in a model of Alzheimer’s disease." Pharmacological Reports 63.5 (2011): 1101-1108.  WB ;  Human.  
      [IF=1.56] Du, Jinghua, et al. "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." Molecular medicine reports 13.3 (2016): 2159-2166.  WB ;  Mouse.  
      [IF=2.94] Wang, Yanqin, et al. "Neuroprotective Effect and Mechanism of Thiazolidinedione on Dopaminergic Neurons In Vivo and In Vitro in Parkinson’s Disease." PPAR Research 2017 (2017).  WB ;  Mouse.  
      [IF=2.19] Zhang, Jin-ming, et al. "Nrf2 Is Crucial For The Down-Regulation Of Cyp7a1 Induced By Arachidonic Acid In Hepg2 Cells." Environmental Toxicology and Pharmacology (2017).  WB ;  Human.  
      [IF=1.83] Li, Gongchu, et al. "Ulva pertusa lectin 1 delivery through adenovirus vector affects multiple signaling pathways in cancer cells." Glycoconjugate Journal (2017): 1-10.  WB ;  Human.  
      [IF=2.33] Ma, Zhihong, et al. "Paeoniflorin suppresses lipid accumulation and alleviates insulin resistance by regulating the Rho kinase/IRS-1 pathway in palmitate-induced HepG2Cells." Biomedicine & Pharmacotherapy 90 (2017): 361-367.  WB ;  Human.  
      [IF=11.33] Leclercq, Sophie, et al. "Low-dose penicillin in early life induces long-term changes in murine gut microbiota, brain cytokines and behavior." Nature Communications 8 (2017): 15062.  WB ;  Mouse.  
      [IF=3.62] Wang, Peng, et al. "Trans-generational enhancement of C-type lysozyme level in eggs of zebrafish by dietary β-glucan." Developmental & Comparative Immunology (2017).  other ;  
      [IF=1.28] Li, Yunlun, et al. "Mechanism of the protective effects of the combined treatment with rhynchophylla total alkaloids and sinapine thiocyanate against a prothrombotic state caused by vascular endothelial cell inflammatory damage." Experimental and Therapeutic Medicine.  WB ;  Mouse.  
      [IF=1.55] Zhang, Zhongti, et al. "Jak3 is involved in CCR7-dependent migration and invasion in metastatic squamous cell carcinoma of the head and neck." Oncology Letters 13.5 (2017): 3191-3197.  WB ;  Human.  
      [IF=2.61] Yang, Yang, et al. "The epigenetically-regulated miR-34a targeting c-SRC suppresses RAF/MEK/ERK signaling pathway in K-562 cells." Leukemia Research 55 (2017): 91-96.  WB ;  Human.  
      [IF=1.66] Xu, Dan-dan, et al. "17β-Estradiol and progesterone decrease MDP induced NOD2 expression in bovine mammary epithelial cells." Veterinary Immunology and Immunopathology (2017).  WB ;  Bovine.  
      [IF=2.58] Huang, Xu, et al. "ICAM-1-Targeted Liposomes Loaded with Liver X Receptor Agonists Suppress PDGF-Induced Proliferation of Vascular Smooth Muscle Cells." Nanoscale Research Letters 12.1 (2017): 322.  WB ;  Mouse.  
      [IF=3.85] Huang, Huimin, et al. "HMGA2, a driver of inflammation, is associated with hypermethylation in acute liver injury." Toxicology and Applied Pharmacology (2017).  WB ;  Mouse.  
      [IF=3.14] Liu, Yaowen, Shuyao Wang, and Rong Zhang. "Composite poly (lactic acid)/chitosan nanofibrous scaffolds for cardiac tissue engineering." International Journal of Biological Macromolecules (2017).  WB ;  Rat.  
      [IF=3.14] Varghese, Sheeja, et al. "The inhibitory effect of anti-tumor polysaccharide from Punica granatum on metastasis." International Journal of Biological Macromolecules (2017).  WB ;  Human.  
      [IF=5.57] Sakaguchi, Takashi, et al. "Regulation of ITGA3 by the dual-stranded microRNA-199 family as a potential prognostic marker in bladder cancer." British journal of cancer 116.8 (2017): 1077.  WB ;  Human.  
      [IF=1.22] Ma, Lili, et al. "Mori cortex prevents kidney damage through inhibiting expression of inflammatory factors in the glomerulus in streptozocin-induced diabetic rats." Iranian Journal of Basic Medical Sciences 20.6 (2017): 715-721.  WB ;  Rat.  
      [IF=2.37] Wang, Mengyuan, et al. "miR-141-3p functions as a tumor suppressor modulating activating transcription factor 5 in glioma." Biochemical and Biophysical Research Communications (2017).  WB ;  Human.  
      [IF=3.98] Lu, Naisheng, et al. "Butyric acid regulates progesterone and estradiol secretion via cAMP signaling pathway in porcine granulosa cells." The Journal of Steroid Biochemistry and Molecular Biology (2017).  WB ;  Pig.  
      [IF=2.05] He, Siyi, et al. "Protective role of downregulated MLK3 in myocardial adaptation to chronic hypoxia." Journal of Physiology and Biochemistry (2017): 1-10.  WB ;  Human.  
      [IF=5.48] Wu, Bin, et al. "Succinate-induced neuronal mitochondrial fission and hexokinase II malfunction in ischemic stroke: Therapeutical effects of kaempferol." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2017).  WB ;  Mouse.  
      [IF=2.16] Wang, Yi Ya, et al. "Epigenetic control of cyp19a1a expression is critical for high temperature induced Nile tilapia masculinization." Journal of Thermal Biology (2017).  WB ;  Other Species.  
      [IF=1.75] Vinothkumar, G., et al. "Abnormal Amyloid β 42 expression and increased oxidative stress in plasma of CKD patients with cognitive dysfunction: A small scale case control study comparison with Alzheimer's disease." BBA Clinical (2017).  WB ;  Human.  
      [IF=3.15] Yang, Kelin, et al. "Phytol promotes the formation of slow-twitch muscle fibers through PGC-1αmiRNA but not mitochondria oxidation." Journal of Agricultural and Food Chemistry (2017).  WB ;  Pig.  
      [IF=2.82] Gao, Ling, et al. "Morusin shows potent antitumor activity for human hepatocellular carcinoma in vitro and in vivo through apoptosis induction and angiogenesis inhibition." Drug Design, Development and Therapy 11 (2017): 1789.  WB ;  Human.  
       

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