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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
SARS-CoV-2 (2019-nCoV) Spike RBD(Y505C)-His Recombinant Protein
- 保质期:
12个月
- 供应商:
北京义翘神州科技股份有限公司
- 保存条件:
-20℃ to -80℃
- 规格:
100 µg
纯度> 90 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Testing in progress
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(Y505C)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(Y505C)) was expressed with a polyhistidine tag at the C-terminus.
NP No.YP_009724390.1
表达宿主HEK293 Cells
种属2019-nCoV
预测 N 端Arg 319
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(Y505C)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.5 kDa. As a result of glycosylation, it migrates as an approximately 35.1 kDa band in SDS-PAGE under reducing conditions.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验日前,北京大学生命科学学院魏文胜课题组在 Cell 杂志上在线发表题为“Circular RNA Vaccines against SARS-CoV-2 and Emerging Variants”的研究论文。 魏文胜团队首先建立了体外高效制备高纯度环状 RNA 的技术平台,针对新型冠状病毒及其变异株,设计了编码新冠病毒刺突蛋白(Spike)受体结构域(RBD)的环状 RNA 疫苗。 该项研究中制备的针对新冠病毒德尔塔变异株的环状 RNA 疫苗(circRNARBD
, Germany) 进行针对SARS-CoV-2 RBD抗体的通用的Antigen-down检测抗体分析,一种采用标准稀释液PBS-T/BSA,另一种采用CANDOR的特色产品(包括LowCross Buffer®作为实验稀释剂)的增强方案,两者进行比较,可以发现亲和力鉴别的积极效果。在阳性样品的最大信号几乎不变的情况下,阴性样品的背景和样品间的变化大大改善,从而获得了更好的检测限。减少背景和阴性样本的变化,同时改善信噪比可以防止许多假阳性结果。如上所述,在SARS-CoV-2抗体检测中,应不惜一切代价避免
5位院士为代表,超12篇CNS大作,5月新冠研究汇总——全方位围攻新冠病毒,值得收藏
的鸡尾酒疗法也引起了大家的关注,不同的抗体可以识别结合域上不同的抗原表位,在未来的临床应用中能够避免免疫逃逸的理想单克隆抗体对。 5 月 8 日,军事医学科学院生物工程研究所陈薇院士团队和西湖大学周强团队强强联合,在预印本平台 bioRxiv 上线题为:A potent neutralizing human antibody reveals the N-terminal domain of the Spike protein of SARS-CoV-2 as a site
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