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- 2025年08月12日
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- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Vaccinia Virus B18R / B19R Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10.00 µg/20.00 µg/100.00 µg
| 规格： | 10.00 µg | 产品价格： | ￥2310.0 |
|---|---|---|---|
| 规格： | 20.00 µg | 产品价格： | ￥3870.0 |
| 规格： | 100.00 µg | 产品价格： | ￥4520.0 |
蛋白名称：Vaccinia virus B18R蛋白, Vaccinia virus B18R protein
蛋白构建：A DNA sequence encoding the B18R (P25213) (Met1-Glu351) was expressed with a C-terminal polyhistidine tag.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：His 20
蛋白分子量：The secreted recombinant B18R consists of 342 amino acids and predicts a molecular mass of 39.8 KDa. The apparent molecular mass of the protein is approximately 46 KDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：P25213
蛋白氨基酸序列：Met1-Glu351
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Working Safely with Vaccinia Virus: Laboratory Technique and the Role of Vaccinia Vaccination
Vaccinia virus, the prototype Orthopoxvirus, is widely used in the laboratory as a model system to study various aspects of viral biology, virus-host interactions, and as a protein expression system and a vaccine vector. The ubiquitous use
Imaging of Vaccinia Virus Entry into HeLa Cells
. The methods presented here describe how to construct a fluorescent recombinant vaccinia virus expressing the core protein A4L fused to mCherry, and how to detect the virus movement on actin-EYFP-expressed HeLa cells.
In-Fusion Cloning with Vaccinia Virus DNA Polymerase
the complementary ends and promotes joint molecule formation. Vaccinia virus single-stranded DNA binding protein can be added to enhance this reaction, although it is not an essential component. The resulting joint molecules are used to transform E. coli
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