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上海玉博生物科技有限公司
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文献和实验Material: Packaging Cells, e.g. Phoenix cells (an adenovirus Ad5-transformed human embryonic kidney cell line 293T, transfected with two MoMLV packaging gene constructs: CMV-Env-PolyA, and RSV-Gag/Pol-Tyt2-PolyA.) See the Nolan lab
Characterization of CGRP Receptor Binding
ligands. Also, some cell lines fail to express CGRP receptors after extensive passage. CGRP binding assays provide a rapid and easy approach for distinguishing between receptors for CGRP and related peptides such as adrenomedullin and amylin.
FACS Procedures for Apoptosis Detection
, although it is very difficult to obtain tight CVs. The protocol below is carried out in 96-well V-bottomed micro titer plates (MTP); with some modifications, it could be carried out in 12 x 75 mm plastic tubes. Phoenix Flow Systems has a protocol for pre-fixing samples
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