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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
) IgG Sepharose 6 Fast Flow (GE Healthcare Life Sciences, cat. no. 17‐0969‐02) Paint shaker (Harbil 5G‐HD) or bead beater (MP bioscience
RNA splicing. One form (IIA) includes and the other form (IIB) excludes a 69-amino acid cysteine-rich globular domain encoded by exon 2 in PIINP. During the process of synthesis, these N-propeptides are removed by specific proteases and released
with solvent. Most proteins fold into globular domains, which have a minimal surface area. Peptides from 10 to 30 kDa typically fold into a single domain. Peptides larger than 50 kDa typically form two or more domains that are independently folded
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