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Purification of Recombinant Protein Derived fkom the Baculovirus Expression System Using Glutathione Affinity Agarose

The baculovirus expression system represents an elegant means whereby high-level expression of foreign genes can be achieved using eukaryotic insect cells (1,2) . Viruses from the Baculoviridae subfamily of insect viruses, usually Autographa

lution of GST-Fusion Protein from Glutathione Agarose

  lution of GST-Fusion Protein from Glutathione Agarose 1. Wash the GST-fusion protein glutathione-agarose complex once with 1% Triton X-100 in PBS. 2. Resuspend the complex in 1% Triton X-100 in PBS. 3. Prepare a small column

Agarose Gel Electrophoresis

实验步骤 1. Dissolving Agarose 1000 ( Method 1: Microwave (recommended for 1) Into a flask holding 2-4 times the desired solution volume, add a magnetic stir bar and the calculated volume of buffer at room temperature.