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文献和实验The baculovirus expression system represents an elegant means whereby high-level expression of foreign genes can be achieved using eukaryotic insect cells (1,2) . Viruses from the Baculoviridae subfamily of insect viruses, usually Autographa
lution of GST-Fusion Protein from Glutathione Agarose
lution of GST-Fusion Protein from Glutathione Agarose 1. Wash the GST-fusion protein glutathione-agarose complex once with 1% Triton X-100 in PBS. 2. Resuspend the complex in 1% Triton X-100 in PBS. 3. Prepare a small column
实验步骤 1. Dissolving Agarose 1000 ( Method 1: Microwave (recommended for 1) Into a flask holding 2-4 times the desired solution volume, add a magnetic stir bar and the calculated volume of buffer at room temperature.
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