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Purification of Recombinant Protein Derived fkom the Baculovirus Expression System Using Glutathione Affinity Agarose

The baculovirus expression system represents an elegant means whereby high-level expression of foreign genes can be achieved using eukaryotic insect cells (1,2) . Viruses from the Baculoviridae subfamily of insect viruses, usually Autographa

lution of GST-Fusion Protein from Glutathione Agarose

  lution of GST-Fusion Protein from Glutathione Agarose 1. Wash the GST-fusion protein glutathione-agarose complex once with 1% Triton X-100 in PBS. 2. Resuspend the complex in 1% Triton X-100 in PBS. 3. Prepare a small column

Introduction to the EMSA (Gel Shift) Technique

or agarose gel electrophoresis. Because the rate of DNA migration is shifted or retarded upon protein binding, the assay is also referred to as a gel shift or gel retardation assay. Until conception of the EMSA by Fried and Crothers3 and Garner and Revzin