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文献和实验of the House Mouse, Mus Musculus Genome number of chromosomes 40 diploid DNA content about 6 pg per cell 3X109 bp per haploid genome recombination units 1600 centimorgans (2000 Kb/cM) approximate number of genes§ 0.5 - 1.0 X 105 percent of genome
Culturing Mouse Embryonic Fibroblasts
to human fibroblasts (Parrinello S., et al. Nature Cell Biol, 2003 ). A. Isolating Primary MEFs: B. Passaging primary MEFS: PROCEDURE Setup mouse breeder pairs in the afternoon. Ideally one male
Methylated DNA Immunoprecipitation (MeDIP)
). A similar antibody is available from other companies (Calbiochem, Diagenode). The antibody should be stored as 20 µl aliquots at –20°C. Magnetic beads Dynabeads M-280 Sheep anti-mouse IgG (Dynal Biotech #112.01
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