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文献和实验Phycoerythrin conjugation protocol
DMSO Dimethyl sulphoxide, anhydrous SPDP 3-(2-pyridyldithio)propionic acid N-hydroxysuccinimide ester (Sigma) SMCC Succinimidyl trans-4-(N-maleimidylmethyl)cyclohexane-1-carboxylate
,R3,R4,R5 7 5.251 1-Butene(1-丁烯-3-炔) 5.5 R4 8 5.323 trans-2-Butene(反-2-丁烯) 0.88 R3,R4,R5 9 5.398 1-Butyne(1-丁炔) 8.07 R4 10 5.548 cis-2-Butene(顺-2-丁烯) 3.72 R3,R4,R5 11 5.950 1,3-Butadinene(1,2-丁二烯) 10.85 R3,R4,R5 12 6.182 3-Methyl-1-butene
of most trans -acting factors requiring access to their specific target sites. Alteration of nucleosome structure and organization is therefore a central feature of gene regulation. Factors gain access to their target sites when the intrinsically dynamic
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