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文献和实验【原创】山寨Stratagene“QuikChange Site-Directed Mutagenesis Kits”实验方案
was according to “QuikChange® Site-Directed Mutagenesis Kit” and “An efficient one-step site-directed and site-saturation mutagenesis protocol”. The melting temperature (Tm) was calculated with the formula given by the protocol of Stratagene 2. Amplification of mutant PCR
Multiple Site-Directed Mutagenesis In Vitro
Polymerase chain reaction (PCR)-based site-directed mutagenesis (SDM) methods are widely used in molecular biology to selectively alter gene sequences (1 ). However, common protocols allow for the introduction
Site-Directed Mutagenesis of Antibody-Variable Regions
oligonucleotide-directed mutants. Several polymerase chain reaction-based mutagenesis techniques have been described (3 –5 ). However, available thermostable polymerases are error-prone, leading to undesired sequence changes. For example, Taq polymerase
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