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文献和实验Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
1) Resuspend the Dynabeads Mouse T-Activator CD3/CD28 in the vial. 2) Transfer the desired volume of Dynabeads to a tube. 3) Add an equal volume of Buffer, or at least 1 ml, and mix (vortex for 5 seconds
实验原理 Dynabeads® CD3⁄CD28 CTS™ offer a simple method for isolation, activation and expansion of human T cells. Firstly, CD3 T cells are separated and concentrated from the apheresis product by magnetic cell separation
Activation and Expansion of Human Treg Cells
to TCR/CD3 and CD28 for full activation and expansion of human Treg cells. 二、实验试剂 2 ml Dynabeads? Human Treg Expander 2 x 107 beads/ml in phosphate buffered saline (PBS), pH 7.4, w/ 0.1% human serum albumin (HSA). Magnet: Any Dynal MPC Buffer
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