agilent特约代理商|200523 QuikChange

【原创】山寨Stratagene“QuikChange Site-Directed Mutagenesis Kits”实验方案

was according to “QuikChange® Site-Directed Mutagenesis Kit” and “An efficient one-step site-directed and site-saturation mutagenesis protocol”. The melting temperature (Tm) was calculated with the formula given by the protocol of Stratagene 2. Amplification of mutant PCR

Site-directed Mutagenesis using PCR

From: Molecular Biology: Current Innovations and Future Trends. Eds. A.M. Griffin and H.G.Griffin. ISBN 1-898486-01-8 1995 Horizon Scientific Press, PO Box 1, Wymondham, Norfolk, U.K.In vitro site-directed mutagenesis is an invaluable technique

Site-Directed Mutagenesis of Antibody-Variable Regions

oligonucleotide-directed mutants. Several polymerase chain reaction-based mutagenesis techniques have been described (3 –5 ). However, available thermostable polymerases are error-prone, leading to undesired sequence changes. For example, Taq polymerase