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上海玉博生物科技有限公司
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文献和实验IMMUNOHISTOCHEMISTRY on PARAFFIN OR CRYOSECTIONS
(starting with slides that have been baked @ 50°C, 30min): 1) Fix in 4% PFA/PBS, 15min 2) rinse with PBS, 3x, 5 min each Proceed to Antigen Retrieval below (step 3) 3) Antigen Retrieval: [Vector Labs: Antigen Unmasking Solution #H3300] For 200 mL: 200mL
) and unrelated proteins by formaldehyde groups. The great majority of monoclonal and polyclonal antibodies used for immunohistochemical (IHC) staining of formalin-fixed, paraffin-embedded (FFPE) tissues necessitate unmasking antigens for antigen retrieval
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
molecule, protein?protein, antigen?antibody, and protein?nucleic acid interactions. It further offers a versatile and adaptable platform amenable to a variety of capture modalities and expression systems, and, most importantly, construction of the array
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