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上海玉博生物科技有限公司
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文献和实验-inactivated)5 mg/ml gentamycin (1:100 of the lab stock)Split 2× per week 1:10. Culture in 10% CO2, 37℃ Materials Neuroprobe 96-well chemotaxis plate 5 mm pore size CAT# ChemoTx# 101-5Favorite chemokine of choice (100 mg/ml)Fixation media: 2.5% FBS
% FBS (not heat-inactivated) 5 mg/ml gentamycin (1:100 of the lab stock) Split 2x per week 1:10. Culture in 10% CO2 , 37o C. Materials Neuroprobe 96-well chemotaxis plate 5 mm pore size CAT# ChemoTx# 101-5 Favorite chemokine of choice
is to prepare the C-Buffer and infuse with CO2 prior to the experiment. 2. The optimal filter pore size varies, depending on the cell type used. Use filter inserts with 3 μm pores for neutrophils and monocytes; for lymphocytes, use 3 to 5 μm pores
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