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文献和实验96-well RNA In Situ Hybridization Protocol
+ 5% Normal Goat Serum + anti-digoxigenin Alkaline Phosphatase (1:2000 dilution), rock 2 hrs at RT. 20. Rinse twice with PBT . 21. Wash embryos 9 X for 10 min. in PBT. 22. Rinse twice with 200ul Alkaline Phosphatase buffer (AP buffer). 23
Preparation of Bacterial Proteins for Analysis by 2D-PAGE
water NOTES: Use Aristar grade Urea from Merck-BDH - or a similar high purity urea. The ampholyte solution currently used is "Resolyte" pH 3.5-10 from Merck-BDH. The buffer requires an alkaline ampholyte solution, which reduces
96-well RNA In Situ Hybridization Protocol
. at RT. 19. Remove PBT, add 200μlPBT + 5% Normal Goat Serum + anti-digoxigenin Alkaline Phosphatase (1:2000 dilution), rock 2 hrs at RT. 20. Rinse twice with PBT . 21. Wash embryos 9 X for 10 min. in PBT. 22. Rinse twice with 200μlAlkaline Phosphatase
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