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文献和实验Vybrant® DyeCycle Green and Orange Stains
, bound to DNA. Materials Required but Not Provided Cells and culture medium Flow cytometer tubes Caution The hazards posed by these stains have not been fully investigated. Since Vybrant® DyeCycle™ Green and Orange
Reagents Medium: 500 ml Dulbecco’s Modified Eagle Medium (Gibco #41966-029) 55 ml FCS (10 %) 2.8 ml Gentamycin Solution (Sigma G-1272, 10 ml)) TrypsinTrypsin (10x) lyophilised (Gibco 25095-019) SolutionVersene
Vybrant® DyeCycle Violet Stain
and culture medium Flow cytometer tubes Caution The hazards posed by this stain have not been fully investigated. Since Vybrant® DyeCycle™ Violet stain is known to bind to nucleic acids, treat the stain as a potential mutagen
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