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文献和实验Gene Expression Analysis on Medium-Density Oligonucleotide Arrays
As the Human Genome Project continues toward its goal of characterizing the genome of human and selected model organisms through complete mapping and sequencing of their DNA, unique opportunities are becoming available for studying genetic
Karthaus, 分享了他们的实验。 一、实验设计 选择三种经常用到的表达系统： Expi293TM – protein expression in HEK293 cells ExpiCHOTM - protein expression in CHO cells ExpiSfTM – protein expression in insect cells 分别在 293，CHO，和 Sf9 细胞中表达 mCherry-His 和 mCherry-Twin-Strep-tag® 蛋白，使用
A Medium or High Throughput Protein Refolding Assay
Expression of insoluble protein in E. coli is a major bottleneck of high throughput structural biology projects. Refolding proteins into native conformations from inclusion bodies could significantly increase the number of protein targets
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