BD特约代理商|551849 Hu IFN-Gma ELIS

Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells

using chromophores to detect the T cell secretion of cytokines were limited to the characterization of a single effector molecule. Notably, studies evaluating the immune response to chronic viral infections often measured IFN- secretion by ELISPOT

Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells

using chromophores to detect the T cell secretion of cytokines were limited to the characterization of a single effector molecule. Notably, studies evaluating the immune response to chronic viral infections often measured IFN- secretion by ELISPOT

ELISPOT标准操作程序（protocol）

对照孔。 4、刺激物：特异性刺激物和非特异性刺激物之分。 5、正对照孔加入10μL PHA，终浓度1-4ug/mL，该浓度能有效刺激IFN-γ的分泌。 6、加入实验者自己的刺激物（用Lympho-Spot™无血清培养基配制成10×终浓度，10μL/well）到实验孔。加完刺激物后不要再拍击ELISPOT板。有人认为拍击板子会让刺激物在孔中混合均匀。实际上，通过扩散，刺激物会很快混合均匀。拍击板子会让细胞聚集分布在孔的外周。 7、加完所有的样品之后，盖上板盖，放入二氧化碳培养箱，37°C培养18-24