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上海玉博生物科技有限公司
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文献和实验(see Note 6 ). Duringthis loading step, the UV monitor may exceed its detectionlimit, which is in part due to the high protein content of thesample (10% FBS) and in part due to the Triton X-100, anonionic detergent containing an aromatic group
Determination of Molecular Size by Zonal Sedimentation Analysis on Sucrose Density Gradients
ingredients for stock solutions (e.g., HEPES, NaCl, Triton X‐100, protease inhibitors, sodium azide) Sucrose, ultrapure Sample
mRNA In Situ Hybridization with Nick-Translated Probes
mRNA In Situ Hybridization with Nick-Translated Probes Cells are fixed with freshly made 4% formaldehyde in PBS, pH 7.4 for 15 min at room temperature. All solutions should be made in Molecular Biology grade ultrapure water (no RNase
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